Cloned (Comment) | Organism |
---|---|
isozyme PpNRH2, phylogenetic analysis, recombinant expression in Escherichia coli in inclusion bodies | Physcomitrium patens |
phylogenetic analysis | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
additional information | functional knockout of the mutant, phenotypic analysis of wild-type and mutant enzymes, overview | Physcomitrium patens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | substrate specificity and kinetics, overview | Zea mays | |
0.06 | - |
adenosine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
0.109 | - |
Xanthosine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
0.111 | - |
adenosine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
0.178 | - |
Xanthosine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
0.468 | - |
uridine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
0.512 | - |
uridine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
0.713 | - |
Inosine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
1.013 | - |
Inosine | pH 7.5, 30°C, recombinant enzyme | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uridine + H2O | Zea mays | - |
D-ribose + uracil | - |
? | |
uridine + H2O | Physcomitrium patens | - |
D-ribose + uracil | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physcomitrium patens | - |
- |
- |
Zea mays | - |
- |
- |
Zea mays | H9D3U7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine + H2O | 15% of the activity with uridine | Zea mays | D-ribose + adenine | - |
? | |
adenosine + H2O | 3.5% of the activity with uridine | Zea mays | D-ribose + adenine | - |
? | |
cytidine + H2O | 3.9% of the activity with uridine | Physcomitrium patens | D-ribose + cytosine | - |
? | |
cytidine + H2O | 5.0% of the activity with uridine | Zea mays | D-ribose + cytosine | - |
? | |
inosine + H2O | 22% of the activity with uridine | Physcomitrium patens | D-ribose + hypoxanthine | - |
? | |
inosine + H2O | 9.3% of the activity with uridine | Zea mays | D-ribose + hypoxanthine | - |
? | |
inosine + H2O | 9.5% of the activity with uridine | Zea mays | D-ribose + hypoxanthine | - |
? | |
additional information | adenosine is a poor substrates, no activity with guanosine, trans-zeatin riboside, and cytokinin riboside, substrate specificity, overview | Physcomitrium patens | ? | - |
? | |
additional information | no or almost no activity with guanosine, trans-zeatin riboside, and cytokinin riboside, substrate specificity, overview | Zea mays | ? | - |
? | |
additional information | poor activity with cytidine, guanosine, trans-zeatin riboside, and cytokinin riboside, substrate specificity, overview | Zea mays | ? | - |
? | |
uridine + H2O | - |
Zea mays | D-ribose + uracil | - |
? | |
uridine + H2O | - |
Physcomitrium patens | D-ribose + uracil | - |
? | |
uridine + H2O | best substrate | Zea mays | D-ribose + uracil | - |
? | |
uridine + H2O | best substrate | Physcomitrium patens | D-ribose + uracil | - |
? | |
xanthosine + H2O | 34% of the activity with uridine | Zea mays | D-ribose + xanthine | - |
? | |
xanthosine + H2O | 53% of the activity with uridine | Zea mays | D-ribose + xanthine | - |
? | |
xanthosine + H2O | 61% of the activity with uridine | Physcomitrium patens | D-ribose + xanthine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PpNRH2 | - |
Physcomitrium patens |
ZmNRH2a | - |
Zea mays |
ZmNRH2b | - |
Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Zea mays |
30 | - |
assay at | Physcomitrium patens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
adenosine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
0.4 | - |
adenosine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
0.61 | - |
Inosine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
1.3 | - |
Xanthosine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
3 | - |
uridine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
4.1 | - |
Inosine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
4.6 | - |
Xanthosine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
23.8 | - |
uridine | pH 7.5, 30°C, recombinant enzyme | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Zea mays |
7.5 | - |
assay at | Physcomitrium patens |
General Information | Comment | Organism |
---|---|---|
malfunction | changes in the levels of purine, pyrimidine, and cytokinin metabolites in knockout mutants, phenotypes, overview | Physcomitrium patens |
additional information | the presence of a tyrosine at position 249 (PpNRH1 numbering) confers high hydrolase activity for purine ribosides | Zea mays |
additional information | the presence of a tyrosine at position 249 (PpNRH1 numbering) confers high hydrolase activity for purine ribosides | Physcomitrium patens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
Inosine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
3 | - |
adenosine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
3.5 | - |
adenosine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
5.7 | - |
Inosine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
6.4 | - |
uridine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
7.5 | - |
Xanthosine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
42 | - |
Xanthosine | pH 7.5, 30°C, recombinant enzyme | Zea mays | |
46 | - |
uridine | pH 7.5, 30°C, recombinant enzyme | Zea mays |