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Literature summary for 3.2.2.3 extracted from
- Purification and properties of uridine hydrolase from mung-bean (Phaseolus radiatus) seedlings (1967), Arch. Biochem. Biophys., 119, 356-362.
General Stability
| General Stability | Organism |
|---|---|
| enzyme retains about 95% of the original activity after dialysis against 0.01 M Tris-HCl buffer, pH 7.5 for 24 h | Vigna radiata var. radiata |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | - |
Vigna radiata var. radiata |  |
| Co2+ | - |
Vigna radiata var. radiata | |
| NEM | - |
Vigna radiata var. radiata | |
| PCMB | reversal by sulfhydryl agents | Vigna radiata var. radiata | |
| Zn2+ | - |
Vigna radiata var. radiata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
uridine | - |
Vigna radiata var. radiata | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme does not require metal ions for activity | Vigna radiata var. radiata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna radiata var. radiata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Vigna radiata var. radiata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Vigna radiata var. radiata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.725 | - |
- |
Vigna radiata var. radiata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uridine + H2O | - |
Vigna radiata var. radiata | uracil + D-ribose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Vigna radiata var. radiata |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | 30°C: about 60% of maximal activity, 60°C: about 35% of maximal activity | Vigna radiata var. radiata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
- |
Vigna radiata var. radiata |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 8 | pH 6.8: about 70% of maximal activity, pH 8.0: about 65% of maximal activity | Vigna radiata var. radiata |
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