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Literature summary for 3.2.2.29 extracted from

  • Hashimoto, H.; Hong, S.; Bhagwat, A.S.; Zhang, X.; Cheng, X.
    Excision of 5-hydroxymethyluracil and 5-carboxylcytosine by the thymine DNA glycosylase domain: its structural basis and implications for active DNA demethylation (2012), Nucleic Acids Res., 40, 10203-10214.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.29

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)-Gold cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with a 28-base pair DNA containing a guanine:5-hydroxymethyluracil mismatch, hanging drop vapor diffusion method, using 30% (w/v) polyethylene glycol 4000, 0.2 M ammonium acetate, 0.1 M sodium acetate, pH 4.6 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5-carboxylcytosine-mismatched double-stranded DNA + H2O Homo sapiens
-
 5-carboxylcytosine + double-stranded DNA with abasic site
-
 ?
5-formylcytosine-mismatched double-stranded DNA + H2O Homo sapiens
-
 5-formylcytosine + double-stranded DNA with abasic site
-
 ?
5-hydroxymethyluracil-mismatched double-stranded DNA + H2O Homo sapiens
-
 5-hydroxymethyluracil + double-stranded DNA with abasic site
-
 ?
thymine-mismatched double-stranded DNA + H2O Homo sapiens
-
 thymine + double-stranded DNA with abasic site
-
 ?
uracil-mismatched double-stranded DNA + H2O Homo sapiens
-
 uracil + double-stranded DNA with abasic site
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q13569
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-charged chelating column chromatography, HiTrap SP column chromatography, and Superdex 75 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-carboxylcytosine-mismatched double-stranded DNA + H2O
-
 Homo sapiens 5-carboxylcytosine + double-stranded DNA with abasic site
-
 ?
5-formylcytosine-mismatched double-stranded DNA + H2O
-
 Homo sapiens 5-formylcytosine + double-stranded DNA with abasic site
-
 ?
5-hydroxymethyluracil-mismatched double-stranded DNA + H2O
-
 Homo sapiens 5-hydroxymethyluracil + double-stranded DNA with abasic site
-
 ?
thymine-mismatched double-stranded DNA + H2O
-
 Homo sapiens thymine + double-stranded DNA with abasic site
-
 ?
uracil-mismatched double-stranded DNA + H2O
-
 Homo sapiens uracil + double-stranded DNA with abasic site
-
 ?

Subunits

Subunits Comment Organism
monomer
-
 Homo sapiens

Synonyms

Synonyms Comment Organism
TDG
-
 Homo sapiens
thymine DNA glycosylase
-
 Homo sapiens