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Literature summary for 3.2.2.29 extracted from

  • Yang, H.; Fitz-Gibbon, S.; Marcotte, E.M.; Tai, J.H.; Hyman, E.C.; Miller, J.H.
    Characterization of a thermostable DNA glycosylase specific for U/G and T/G mismatches from the hyperthermophilic archaeon Pyrobaculum aerophilum (2000), J. Bacteriol., 182, 1272-1279.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.29

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrobaculum aerophilum

Metals/Ions

Metals/Ions Comment Organism Structure
4Fe-4S cluster the enzyme contains a binding motif for the 4Fe-4S cluster Pyrobaculum aerophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27900
-
 mass spectrometry Pyrobaculum aerophilum

Organism

Organism UniProt Comment Textmining
Pyrobaculum aerophilum Q9P9L6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrobaculum aerophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thymine-mismatched double-stranded DNA + H2O removes thymine from T/G mismatches Pyrobaculum aerophilum thymine + double-stranded DNA with abasic site
-
 ?
uracil-mismatched double-stranded DNA + H2O removes uracil from G/U mismatches, U/7,8-dihydro-oxoguanine and T/7,8-dihydro-oxoguanine mismatches Pyrobaculum aerophilum uracil + double-stranded DNA with abasic site
-
 ?

Synonyms

Synonyms Comment Organism
Pa-MIG
-
 Pyrobaculum aerophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 10 min, 87% of the protein remains soluble Pyrobaculum aerophilum
90
-
 10 min, 56% of the protein remains soluble Pyrobaculum aerophilum