Cloned (Comment) | Organism |
---|---|
expression in NIH3T3 fibroblasts | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Mus musculus | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
cellular TDG demonstrates PMA-dependent alterations in apparent molecular weight and isoelectric point | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | TDG performs DNA processing of G:T mispairs | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
acetylation | TDG lysines are acetylated by CREB-binding protein, CBP, and p300. Acetylation of the N-terminal region abrogates high-affinity DNA binding and selectively prevents processing of G:T mispairs. TDG acetylation and phosphorylation are mutually exclusive, and their interplay dramatically alters the DNA mispair-processing functions of TDG | Mus musculus |
phosphoprotein | protein kinase C alpha interacts with TDG and phosphorylates N-terminal serine residues adjacent to lysines acetylated by CREB-binding protein, CBP, and p300. TDG acetylation and phosphorylation are mutually exclusive, and their interplay dramatically alters the DNA mispair-processing functions of TDG. Phosphorylation does not markedly alter DNA interactions, but may preserve G:T processing in vivo by preventing CBP-mediated acetylation | Mus musculus |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from NIH3T3 fibroblasts | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
NIH-3T3 cell | - |
Mus musculus | - |
P-19 cell | EC cell | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | TDG performs DNA processing of G:T mispairs | Mus musculus | ? | - |
? | |
additional information | TDG performs DNA processing of G:T mispairs, base excision from oligonucleotides containing a single G:U or G:T mispair | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TDG | - |
Mus musculus |
thymine DNA glycosylase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Mus musculus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mus musculus | cellular TDG demonstrates PMA-dependent alterations in apparent molecular weight and isoelectric point | - |
additional information |
General Information | Comment | Organism |
---|---|---|
additional information | opposing roles of CBP/p300 and PKCalpha in regulating the DNA repair functions of TDG, the interplay of acetylation and phosphorylation of TDG in vivo may be critically important in the maintenance of CpG dinucleotides and epigenetic regulation | Mus musculus |
physiological function | TDG plays an integral role in CpG maintenance by excising mispaired thymine and uracil in a CpG context and also participates in transcriptional regulation via gene-specific CpG demethylation and functional interactions with the transcription machinery | Mus musculus |