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Literature summary for 3.2.2.29 extracted from
- The versatile thymine DNA-glycosylase: a comparative characterization of the human, Drosophila and fission yeast orthologs (2003), Nucleic Acids Res., 31, 2261-2271.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Thd1, phylogenetic analysis, functional expression of a His-tagged truncated variant comprising residues 650M-1063N in Escherichia coli strain BL21 (DE3) | Drosophila melanogaster |
| phylogenetic analysis, expression of His-tagged TDG in Escherichia coli strain BL21 (DE3) | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a His-tagged truncated Thd1p variant comprising residues 650M-1063N from Escherichia coli strain BL21 (DE3) | Drosophila melanogaster |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
x * 46000, recombinant enzyme, SDS-PAGE | Homo sapiens |
| 60000 | - |
x * 60000, recombinant enzyme, SDS-PAGE | Drosophila melanogaster |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| thymine-mismatched double-stranded DNA + H2O | Homo sapiens | the human enzyme excises thymine and uracil from G-T and G-U mismatches, respectively, and is therefore proposed to play a central role in the cellular defense against genetic mutation through spontaneous deamination of 5-methylcytosine and cytosine | thymine + double-stranded DNA with abasic site | - |
? |  |
| uracil-mismatched double-stranded DNA + H2O | Drosophila melanogaster | - |
uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | Homo sapiens | the human enzyme excises thymine and uracil from G-T and G-U mismatches, respectively, and is therefore proposed to play a central role in the cellular defense against genetic mutation through spontaneous deamination of 5-methylcytosine and cytosine | uracil + double-stranded DNA with abasic site | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila melanogaster | Q9V4D8 | - |
- |
| Homo sapiens | Q13569 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged TDG from Escherichia coli strain BL21 (DE3) | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
glycosylase activity of the recombinant enzyme, differential G-T processing, overview | Drosophila melanogaster |
| additional information | - |
glycosylase activity of the recombinant enzyme, differential G-T processing, overview | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3,N4-ethenocytosine-mismatched double-stranded DNA + H2O | - |
Drosophila melanogaster | 3,N4-ethenocytosine + double-stranded DNA with abasic site | - |
? | |
| 5-bromouracil-mismatched double-stranded DNA + H2O | paired with guanine | Homo sapiens | 5-bromouracil + double-stranded DNA with abasic site | - |
? | |
| 5-fluorouracil-mismatched double-stranded DNA + H2O | - |
Drosophila melanogaster | 5-fluorouracil + double-stranded DNA with abasic site | - |
? | |
| 5-fluorouracil-mismatched double-stranded DNA + H2O | paired with guanine | Homo sapiens | 5-fluorouracil + double-stranded DNA with abasic site | - |
? | |
| 5-hydroxymethyluracil-mismatched double-stranded DNA + H2O | paired with guanine | Homo sapiens | 5-hydroxymethyluracil + double-stranded DNA with abasic site | - |
? | |
| 5-methylcytosine-mismatched double-stranded DNA + H2O | - |
Drosophila melanogaster | 5-methylcytosine + double-stranded DNA with abasic site | - |
? | |
| 5-methylcytosine-mismatched double-stranded DNA + H2O | paired with guanine | Homo sapiens | 5-methylcytosine + double-stranded DNA with abasic site | - |
? | |
| cytosine-mismatched double-stranded DNA + H2O | paired with guanine | Homo sapiens | cytosine + double-stranded DNA with abasic site | - |
? | |
| additional information | human TDG removes the substrate pyrimidines 5-fluorouracil, 5-hydroxymethyluracil, 5-bromouracil, epsilonC and thymine paired with guanine with high relative efficiencies, substrate spectrum and substrate binding structure, overview | Homo sapiens | ? | - |
? | |
| additional information | the enzyme shows a broad and species-specific substrate spectrum, substrate binding structure, overview. The common most efficiently processed substrates of all are uracil and 3,N4-ethenocytosine opposite guanine and 5-fluorouracil in any double-stranded DNA context, the enzyme is able to hydrolyze a non-damaged 5'-methylcytosine opposite G, and the double strand and mismatch dependency of the enzymes varies with the substrate | Drosophila melanogaster | ? | - |
? | |
| thymine-mismatched double-stranded DNA + H2O | the human enzyme excises thymine and uracil from G-T and G-U mismatches, respectively, and is therefore proposed to play a central role in the cellular defense against genetic mutation through spontaneous deamination of 5-methylcytosine and cytosine | Homo sapiens | thymine + double-stranded DNA with abasic site | - |
? | |
| thymine-mismatched double-stranded DNA + H2O | G-T mismatch is only a poor substrate for Thd1p | Drosophila melanogaster | thymine + double-stranded DNA with abasic site | - |
? | |
| thymine-mismatched double-stranded DNA + H2O | paired with guanine | Homo sapiens | thymine + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | - |
Drosophila melanogaster | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | - |
Homo sapiens | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | the human enzyme excises thymine and uracil from G-T and G-U mismatches, respectively, and is therefore proposed to play a central role in the cellular defense against genetic mutation through spontaneous deamination of 5-methylcytosine and cytosine | Homo sapiens | uracil + double-stranded DNA with abasic site | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, recombinant enzyme, SDS-PAGE | Drosophila melanogaster |
| ? | x * 46000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the uracil-DNA glycosylase family | Homo sapiens |
| TDG | - |
Homo sapiens |
| Thd1p | - |
Drosophila melanogaster |
| thymine DNA-glycosylase | - |
Drosophila melanogaster |
| thymine DNA-glycosylase | - |
Homo sapiens |
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