Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-bromouracil-mismatched double-stranded DNA + H2O | Homo sapiens | potential role played by human TDG in the cytotoxic effects of 5-chlorouracil and 5-bromouracil incorporation into DNA, which can occur under inflammatory conditions | 5-bromouracil + double-stranded DNA with abasic site | - |
? | |
5-chlorouracil-mismatched double-stranded DNA + H2O | Homo sapiens | potential role played by human TDG in the cytotoxic effects of 5-chlorouracil and 5-bromouracil incorporation into DNA, which can occur under inflammatory conditions | 5-chlorouracil + double-stranded DNA with abasic site | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q13569 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-bromouracil-mismatched double-stranded DNA + H2O | potential role played by human TDG in the cytotoxic effects of 5-chlorouracil and 5-bromouracil incorporation into DNA, which can occur under inflammatory conditions | Homo sapiens | 5-bromouracil + double-stranded DNA with abasic site | - |
? | |
5-chlorouracil-mismatched double-stranded DNA + H2O | potential role played by human TDG in the cytotoxic effects of 5-chlorouracil and 5-bromouracil incorporation into DNA, which can occur under inflammatory conditions | Homo sapiens | 5-chlorouracil + double-stranded DNA with abasic site | - |
? | |
5-chlorouracil-mismatched double-stranded DNA + H2O | removes a variety of damaged bases (X) with a preference for lesions in a CpG/X context. The maximal activity for G/X substrates depends significantly on the 5' base pair. The maximal activity decreases by 6fold, 11fold, and 82fold for TpG/5-chlorouracil, GpG/5-chlorouracil, and ApG/5-chlorouracil, respectively, as compared with CpG/5-chlorouracil. Human TDG activity is reduced 102.3104.3fold for A/X relative to G/X pairs and reduced further for A/X pairs with a 5' pair other than C/G. The effect of altering the 5' pair and/or the opposing base (G/X versus A/X) is greater for substrates that are larger (bromodeoxyuridine, dT) or have a more stable N-glycosidic bond (such as dT). The largest CpG context effects are observed for the excision of thymine | Homo sapiens | 5-chlorouracil + double-stranded DNA with abasic site | - |
? | |
5-fluorouracil-mismatched double-stranded DNA + H2O | the activity for G/5-fluorouracil, G/5-chlorouracil, and G/5-bromouracil, with any 5'-flanking pair, meets and in most cases significantly exceeds the CpG/T activity. Human TDG activity is reduced 102.3104.3fold for A/X relative to G/X pairs and reduced further for A/X pairs with a 5' pair other than C/G. The effect of altering the 5' pair and/or the opposing base (G/X versus A/X) is greater for substrates that are larger (bromodeoxyuridine, dT) or have a more stable N-glycosidic bond (such as dT). The largest CpG context effects are observed for the excision of thymine | Homo sapiens | 5-fluorouracil + double-stranded DNA with abasic site | - |
? | |
thymine-mismatched double-stranded DNA + H2O | thymine DNA glycosylase excises thymine from G/T mispairs. Human TDG activity is reduced 102.3104.3fold for A/X relative to G/X pairs and reduced further for A/X pairs with a 5' pair other than C/G. The effect of altering the 5' pair and/or the opposing base (G/X versus A/X) is greater for substrates that are larger (bromodeoxyuridine, dT) or have a more stable N-glycosidic bond (such as dT). The largest CpG context effects are observed for the excision of thymine | Homo sapiens | thymine + double-stranded DNA with abasic site | - |
? |
Synonyms | Comment | Organism |
---|---|---|
hTDG | - |
Homo sapiens |
thymine DNA glycosylase | - |
Homo sapiens |