Any feedback?
Literature summary for 3.2.2.28 extracted from
- Specificity and catalytic mechanism in family 5 uracil DNA glycosylase (2014), J. Biol. Chem., 289, 18413-18426.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BH214 cells | Thermus thermophilus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D75A | the mutant loses xanthine DNA glycosylase activity while retaining partial uracil and hypoxanthine DNA glycosylase activity | Thermus thermophilus |
| D75N | the mutant loses xanthine DNA glycosylase activity while retaining much of their uracil and hypoxanthine DNA glycosylase activity | Thermus thermophilus |
| D75Q | the mutant loses xanthine DNA glycosylase activity while retaining much of their uracil and hypoxanthine DNA glycosylase activity | Thermus thermophilus |
| H190A | inactive | Thermus thermophilus |
| N120A | the mutant loses xanthine DNA glycosylase activity while retaining partial uracil and hypoxanthine DNA glycosylase activity | Thermus thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by Ugi peptide | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hypoxanthine-mismatched double-stranded DNA + H2O | Thermus thermophilus | the enzyme also acts as a hypoxanthine DNA glycosylase with the strongest activity on the G/I base pair but no activity detected on the C/I base pair | hypoxanthine + double-stranded DNA with abasic site | - |
? |  |
| hypoxanthine-mismatched double-stranded DNA + H2O | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | the enzyme also acts as a hypoxanthine DNA glycosylase with the strongest activity on the G/I base pair but no activity detected on the C/I base pair | hypoxanthine + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | Thermus thermophilus | the enzyme acts as a double-stranded uracil DNA glycosylase with a relatively low activity on the A/U base pair | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | the enzyme acts as a double-stranded uracil DNA glycosylase with a relatively low activity on the A/U base pair | uracil + double-stranded DNA with abasic site | - |
? | |
| xanthine-mismatched double-stranded DNA + H2O | Thermus thermophilus | - |
xanthine + double-stranded DNA with abasic site | - |
? | |
| xanthine-mismatched double-stranded DNA + H2O | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
xanthine + double-stranded DNA with abasic site | - |
? | |
| xanthine-mismatched single-stranded DNA + H2O | Thermus thermophilus | - |
xanthine + single-stranded DNA with abasic site | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermus thermophilus | Q5SJ65 | - |
- |
| Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | Q5SJ65 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiTrap chelating column chromatography | Thermus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hypoxanthine-mismatched double-stranded DNA + H2O | the enzyme also acts as a hypoxanthine DNA glycosylase with the strongest activity on the G/I base pair but no activity detected on the C/I base pair | Thermus thermophilus | hypoxanthine + double-stranded DNA with abasic site | - |
? | |
| hypoxanthine-mismatched double-stranded DNA + H2O | the enzyme also acts as a hypoxanthine DNA glycosylase with the strongest activity on the G/I base pair but no activity detected on the C/I base pair | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | hypoxanthine + double-stranded DNA with abasic site | - |
? | |
| additional information | the enzyme showed no activity on oxanine-containing DNA substrates, uracil-mismatched single-stranded DNA and hypoxanthine-mismatched single-stranded DNA | Thermus thermophilus | ? | - |
? | |
| additional information | the enzyme showed no activity on oxanine-containing DNA substrates, uracil-mismatched single-stranded DNA and hypoxanthine-mismatched single-stranded DNA | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | the enzyme acts as a double-stranded uracil DNA glycosylase with a relatively low activity on the A/U base pair | Thermus thermophilus | uracil + double-stranded DNA with abasic site | - |
? | |
| uracil-mismatched double-stranded DNA + H2O | the enzyme acts as a double-stranded uracil DNA glycosylase with a relatively low activity on the A/U base pair | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | uracil + double-stranded DNA with abasic site | - |
? | |
| xanthine-mismatched double-stranded DNA + H2O | - |
Thermus thermophilus | xanthine + double-stranded DNA with abasic site | - |
? | |
| xanthine-mismatched double-stranded DNA + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | xanthine + double-stranded DNA with abasic site | - |
? | |
| xanthine-mismatched single-stranded DNA + H2O | - |
Thermus thermophilus | xanthine + single-stranded DNA with abasic site | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| family 5 UDGb | - |
Thermus thermophilus |
| family 5 uracil DNA glycosylase | - |
Thermus thermophilus |
| UDG | - |
Thermus thermophilus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
