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Literature summary for 3.2.2.28 extracted from

  • Saparbaev, M.; Laval, J.
    3,N4-ethenocytosine, a highly mutagenic adduct, is a primary substrate for Escherichia coli double-stranded uracil-DNA glycosylase and human mismatch-specific thymine-DNA glycosylase (1998), Proc. Natl. Acad. Sci. USA, 95, 8508-8513.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000025
-
3,N4-ethenocytosine-mismatched double-stranded DNA
-
Escherichia coli
0.0000227
-
uracil-mismatched double-stranded DNA
-
Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,N4-ethenocytosine-mismatched double-stranded DNA + H2O 3,N4-ethenocytosine is recognized and efficiently excised from the 3,N4-ethenocytosine/G mismatch Escherichia coli 3,N4-ethenocytosine + double-stranded DNA with abasic site
-
?
additional information the excision of thymine in a G/T mismatch by dsUDG is extremely low. This enzymatic activity does not have a real biological significance Escherichia coli ?
-
?
uracil-mismatched double-stranded DNA + H2O excision of uracil from U/G mismatch Escherichia coli uracil + double-stranded DNA with abasic site
-
?

Synonyms

Synonyms Comment Organism
double-stranded uracil-DNA glycosylase
-
Escherichia coli
dsUDG
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0028
-
uracil-mismatched double-stranded DNA
-
Escherichia coli
0.016
-
3,N4-ethenocytosine-mismatched double-stranded DNA
-
Escherichia coli