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Literature summary for 3.2.2.28 extracted from

  • O'Neill, R.J.; Vorobeva, O.V.; Shahbakhti, H.; Zmuda, E.; Bhagwat, A.S.; Baldwin, G.S.
    Mismatch uracil glycosylase from Escherichia coli: a general mismatch or a specific DNA glycosylase? (2003), J. Biol. Chem., 278, 20526-20532.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A9H1
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-

Purification (Commentary)

Purification (Comment) Organism
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Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,N4-ethenocytosine-containing single-stranded DNA + H2O the enzyme excised both 3,N4-ethenocytosine and uracil from DNA. 3,N4-ethenocytosine is significantly better as a substrate in terms of binding and hydrolysis. The tighter binding of the 3,N4-ethenocytosine containing substrate by MUG probably also accounts for its activity against single-stranded DNA containing 3,N4-ethenocytosine. Cleavage of the single-stranded substrate is 1500fold slower than the double-stranded substrate Escherichia coli 3,N4-ethenocytosine + single-stranded DNA with abasic site
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?
3,N4-ethenocytosine-mismatched double-stranded DNA + H2O the enzyme excised both 3,N4-ethenocytosine and uracil from DNA. 3,N4-ethenocytosine is significantly better as a substrate in terms of binding and hydrolysis. The tighter binding of the 3,N4-ethenocytosine containing substrate by MUG probably also accounts for its activity against single-stranded DNA containing 3,N4-ethenocytosine. Cleavage of the single-stranded substrate is 1500fold slower than the double-stranded substrate. Of the different substrates tested, a duplex containing the 3,N4-ethenocytosine pair has the highest affinity for the enzyme, U/G is the next best substrate Escherichia coli 3,N4-ethenocytosine + double-stranded DNA with abasic site
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?
additional information 5-Hydroxyuracil and inosine (hypoxanthine) show cleavage rates 2–3 orders of magnitude slower than 3,N4-ethenocytosine. Thymine, 5-hydroxymethyluracil, and 5-hydroxycytosine are cleaved to some extent, although extremely slowly Escherichia coli ?
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?
uracil-mismatched double-stranded DNA + H2O the enzyme activity against the uracil-containing single-stranded DNA is so low that it is not likely to be of any significance. Of the different substrates tested, a duplex containing the 3,N4-ethenocytosine pair has the highest affinity for the enzyme, U/G is the next best substrate Escherichia coli uracil + double-stranded DNA with abasic site
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?

Synonyms

Synonyms Comment Organism
MUG
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Escherichia coli