Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A9H1 | - |
- |
Purification (Comment) | Organism |
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- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
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3,N4-ethenocytosine-containing single-stranded DNA + H2O | the enzyme excised both 3,N4-ethenocytosine and uracil from DNA. 3,N4-ethenocytosine is significantly better as a substrate in terms of binding and hydrolysis. The tighter binding of the 3,N4-ethenocytosine containing substrate by MUG probably also accounts for its activity against single-stranded DNA containing 3,N4-ethenocytosine. Cleavage of the single-stranded substrate is 1500fold slower than the double-stranded substrate | Escherichia coli | 3,N4-ethenocytosine + single-stranded DNA with abasic site | - |
? | |
3,N4-ethenocytosine-mismatched double-stranded DNA + H2O | the enzyme excised both 3,N4-ethenocytosine and uracil from DNA. 3,N4-ethenocytosine is significantly better as a substrate in terms of binding and hydrolysis. The tighter binding of the 3,N4-ethenocytosine containing substrate by MUG probably also accounts for its activity against single-stranded DNA containing 3,N4-ethenocytosine. Cleavage of the single-stranded substrate is 1500fold slower than the double-stranded substrate. Of the different substrates tested, a duplex containing the 3,N4-ethenocytosine pair has the highest affinity for the enzyme, U/G is the next best substrate | Escherichia coli | 3,N4-ethenocytosine + double-stranded DNA with abasic site | - |
? | |
additional information | 5-Hydroxyuracil and inosine (hypoxanthine) show cleavage rates 23 orders of magnitude slower than 3,N4-ethenocytosine. Thymine, 5-hydroxymethyluracil, and 5-hydroxycytosine are cleaved to some extent, although extremely slowly | Escherichia coli | ? | - |
? | |
uracil-mismatched double-stranded DNA + H2O | the enzyme activity against the uracil-containing single-stranded DNA is so low that it is not likely to be of any significance. Of the different substrates tested, a duplex containing the 3,N4-ethenocytosine pair has the highest affinity for the enzyme, U/G is the next best substrate | Escherichia coli | uracil + double-stranded DNA with abasic site | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MUG | - |
Escherichia coli |