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Literature summary for 3.2.2.27 extracted from

  • Chembazhi, U.V.; Patil, V.V.; Sah, S.; Reeve, W.; Tiwari, R.P.; Woo, E.; Varshney, U.
    Uracil DNA glycosylase (UDG) activities in Bradyrhizobium diazoefficiens characterization of a new class of UDG with broad substrate specificity (2017), Nucleic Acids Res., 45, 5863-5876 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.27

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain CC102 Bradyrhizobium diazoefficiens

Protein Variants

Protein Variants Comment Organism
A59Y the mutation leads to a decrease in its activity on xanthine, and 5-hydroxymethyluracil containing single stranded DNAs but not on uracil containing single stranded DNA Bradyrhizobium diazoefficiens

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by ctcaagtg-tetrahydrofuran-aggcatgcaagagct, DNA with abasic site, and Ugi protein Bradyrhizobium diazoefficiens
Uracil free uracil causes a 5fold reduction in enzyme activity at 10 mM concentration Bradyrhizobium diazoefficiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uracil-containing single-stranded DNA + H2O Bradyrhizobium diazoefficiens
-
 uracil + single-stranded DNA with abasic site
-
 ?
uracil-containing single-stranded DNA + H2O Bradyrhizobium diazoefficiens USDA110
-
 uracil + single-stranded DNA with abasic site
-
 ?
uracil-mismatched double-stranded DNA + H2O Bradyrhizobium diazoefficiens
-
 uracil + double-stranded DNA with abasic site
-
 ?
uracil-mismatched double-stranded DNA + H2O Bradyrhizobium diazoefficiens USDA110
-
 uracil + double-stranded DNA with abasic site
-
 ?

Organism

Organism UniProt Comment Textmining
Bradyrhizobium diazoefficiens Q89XR0 previously Bradyrhizobium japonicum
-
Bradyrhizobium diazoefficiens USDA110 Q89XR0 previously Bradyrhizobium japonicum
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Bradyrhizobium diazoefficiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxymethyluracil-containing single-stranded DNA + H2O
-
 Bradyrhizobium diazoefficiens 5-hydroxymethyluracil + single-stranded DNA with abasic site
-
 ?
hydroxymethyluracil-containing single-stranded DNA + H2O
-
 Bradyrhizobium diazoefficiens USDA110 5-hydroxymethyluracil + single-stranded DNA with abasic site
-
 ?
additional information the enzyme does not utilize DNA oligomer containing inosine or thymine in single stranded DNA or double stranded DNA Bradyrhizobium diazoefficiens ?
-
 ?
additional information the enzyme prefers single stranded DNA Bradyrhizobium diazoefficiens ?
-
 ?
additional information the enzyme does not utilize DNA oligomer containing inosine or thymine in single stranded DNA or double stranded DNA Bradyrhizobium diazoefficiens USDA110 ?
-
 ?
additional information the enzyme prefers single stranded DNA Bradyrhizobium diazoefficiens USDA110 ?
-
 ?
uracil-containing single-stranded DNA + H2O
-
 Bradyrhizobium diazoefficiens uracil + single-stranded DNA with abasic site
-
 ?
uracil-containing single-stranded DNA + H2O
-
 Bradyrhizobium diazoefficiens USDA110 uracil + single-stranded DNA with abasic site
-
 ?
uracil-guanine-mismatched double-stranded DNA + H2O
-
 Bradyrhizobium diazoefficiens uracil + double-stranded DNA with abasic site
-
 ?
uracil-mismatched double-stranded DNA + H2O
-
 Bradyrhizobium diazoefficiens uracil + double-stranded DNA with abasic site
-
 ?
uracil-mismatched double-stranded DNA + H2O
-
 Bradyrhizobium diazoefficiens USDA110 uracil + double-stranded DNA with abasic site
-
 ?
xanthine-containing single-stranded DNA + H2O
-
 Bradyrhizobium diazoefficiens xanthine + single-stranded DNA with abasic site
-
 ?

Synonyms

Synonyms Comment Organism
Blr0248
-
 Bradyrhizobium diazoefficiens
UDG
-
 Bradyrhizobium diazoefficiens
UNG
-
 Bradyrhizobium diazoefficiens
uracil DNA glycosylase
-
 Bradyrhizobium diazoefficiens