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Literature summary for 3.2.2.27 extracted from

  • Yi, G.S.; Wang, W.W.; Cao, W.G.; Wang, F.P.; Liu, X.P.
    Sulfolobus acidocaldarius UDG can remove dU from the RNA backbone insight into the specific recognition of uracil linked with deoxyribose (2017), Genes (Basel), 8, E38 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.27

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta2(DE3)pLysS cells Sulfolobus acidocaldarius

Inhibitors

Inhibitors Comment Organism Structure
Co2+
-
 Sulfolobus acidocaldarius
Cu2+
-
 Sulfolobus acidocaldarius
NaCl when the concentration of NaCl is higher than 50 mM, the enzymatic activity is decreased by more than 50% Sulfolobus acidocaldarius
Ni2+
-
 Sulfolobus acidocaldarius
Zn2+
-
 Sulfolobus acidocaldarius

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected by Mg2+ and Mn2+ Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius
-
-
-
Sulfolobus acidocaldarius DSM639
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex S200 gel filtration Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxyuracil-containing single-stranded DNA + H2O
-
 Sulfolobus acidocaldarius deoxyuracil + single-stranded DNA with abasic site
-
 ?
deoxyuracil-containing single-stranded DNA + H2O
-
 Sulfolobus acidocaldarius DSM639 deoxyuracil + single-stranded DNA with abasic site
-
 ?
deoxyuracil-containing single-stranded RNA + H2O
-
 Sulfolobus acidocaldarius deoxyuracil + single-stranded RNA
-
 ?
deoxyuracil-containing single-stranded RNA + H2O
-
 Sulfolobus acidocaldarius DSM639 deoxyuracil + single-stranded RNA
-
 ?
deoxyuracil-mismatched double-stranded DNA + H2O isoform UDGIV removes deoxyuracil from double-stranded DNA in the following order of efficiency C/U = G/U > T/U > A/U. The preference order of double stranded DNA is G/U > C/U = T/U = A/U for isoform UDGV Sulfolobus acidocaldarius deoxyuracil + double-stranded DNA with abasic site
-
 ?
deoxyuracil-mismatched double-stranded DNA + H2O isoform UDGIV removes deoxyuracil from double-stranded DNA in the following order of efficiency C/U = G/U > T/U > A/U. The preference order of double stranded DNA is G/U > C/U = T/U = A/U for isoform UDGV Sulfolobus acidocaldarius DSM639 deoxyuracil + double-stranded DNA with abasic site
-
 ?
additional information the removal of ribose-linked uracil from DNA backbone is inefficient. The enzyme cannot remove 2,2'-anhydro uridine, hypoxanthine, and 7-deazaxanthine from single-stranded DNA and single-stranded DNA Sulfolobus acidocaldarius ?
-
 ?
additional information the removal of ribose-linked uracil from DNA backbone is inefficient. The enzyme cannot remove 2,2'-anhydro uridine, hypoxanthine, and 7-deazaxanthine from single-stranded DNA and single-stranded DNA Sulfolobus acidocaldarius DSM639 ?
-
 ?

Synonyms

Synonyms Comment Organism
UDGIV isoform Sulfolobus acidocaldarius
UDGV isoform Sulfolobus acidocaldarius

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 85 high activity between 55 and 85°C Sulfolobus acidocaldarius

Cofactor

Cofactor Comment Organism Structure
[4Fe-4S]-center
-
 Sulfolobus acidocaldarius