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Literature summary for 3.2.2.27 extracted from

  • Arif, S.M.; Geethanandan, K.; Mishra, P.; Surolia, A.; Varshney, U.; Vijayan, M.
    Structural plasticity in Mycobacterium tuberculosis uracil-DNA glycosylase (MtUng) and its functional implications (2015), Acta Crystallogr. Sect. D, 71, 1514-1527 .
    View publication on PubMed
Search for more literature for 3.2.2.27

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in complex with 5-chlorouracil, microbatch-under-oil method, using 0.15 M potassium bromide, 30%(w/v) polyethylene glycol monomethyl ether 2000 Mycobacterium tuberculosis
enzyme in complex with 5-fluorouracil, microbatch-under-oil method, using 0.2 M sodium acetate trihydrate, 0.1 MTris-HCl pH 8.5, 30%(w/v) PEG 4000, 4%(v/v) 1,3-butanediol Mycobacterium tuberculosis
enzyme in complex with uracil or 5-nitrouracil, microbatch-under-oil method, using 0.2 M sodium acetate trihydrate, 0.1 M sodium cacodylate trihydrate pH 6.5, 30%(w/v) PEG 8000, 5%(v/v) Jeffamine M-600 pH 7.0 Mycobacterium tuberculosis
enzyme in complex with uracil, microbatch-under-oil method, using 0.2 M ammonium acetate, 0.1 M sodium acetate trihydrate pH 4.6, 30%(w/v) PEG 4000, 4%(v/v) 1,1,1,3,3,3-hexafluoro-2-propanol Mycobacterium tuberculosis
native enzyme and enzyme in complex with 2-thiouracil, microbatch-under-oil method, using 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6, 30%(w/v) PEG 4000 Mycobacterium tuberculosis
native enzyme and enzyme in complex with 5-fluorouracil, 5-nitrouracil, 5-aminouracil or 6-aminouracil, microbatch-under-oil method, using 1.6 M sodium citrate tribasic dihydrate pH 6.5 Mycobacterium tuberculosis

Inhibitors

Inhibitors Comment Organism Structure
uracil-DNA glycosylase inhibitor Ugi Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uracil-containing single-stranded DNA + H2O Mycobacterium tuberculosis
-
 uracil + single-stranded DNA with abasic site
-
 ?
uracil-mismatched double-stranded DNA + H2O Mycobacterium tuberculosis
-
 uracil + double-stranded DNA with abasic site
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
uracil-containing single-stranded DNA + H2O
-
 Mycobacterium tuberculosis uracil + single-stranded DNA with abasic site
-
 ?
uracil-mismatched double-stranded DNA + H2O
-
 Mycobacterium tuberculosis uracil + double-stranded DNA with abasic site
-
 ?

Synonyms

Synonyms Comment Organism
UNG
-
 Mycobacterium tuberculosis