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Literature summary for 3.2.2.27 extracted from
- A mutant of uracil DNA glycosylase that distinguishes between cytosine and 5-methylcytosine (2014), PLoS ONE, 9, e95394.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3)pLysS cells | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| N123D/L191A | the mutation generates an enzyme that excises cytosine and distinguishes between cytosine and methylcytosine | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil-mismatched double-stranded DNA + H2O | Escherichia coli | - |
uracil + double-stranded DNA with abasic site | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P12295 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil-mismatched double-stranded DNA + H2O | - |
Escherichia coli | uracil + double-stranded DNA with abasic site | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDG | - |
Escherichia coli |
| uracil DNA glycosylase | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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