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Literature summary for 3.2.2.27 extracted from

  • Lee, D.H.; Liu, Y.; Lee, H.W.; Xia, B.; Brice, A.R.; Park, S.H.; Balduf, H.; Dominy, B.N.; Cao, W.
    A structural determinant in the uracil DNA glycosylase superfamily for the removal of uracil from adenine/uracil base pairs (2015), Nucleic Acids Res., 2, 1081-1089.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.27

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BH214 cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
N123A the mutation substantially dramatically reduces enzyme activity on A/U base pairs and other double-stranded uracil-containing base pairs Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uracil-mismatched double-stranded DNA + H2O Escherichia coli family 2 mismatch-specific uracil DNA glycosylase (MUG) is known to exhibit glycosylase activity on three mismatched base pairs, T/U, G/U and C/U. Family 1 uracil N-glycosylase (UNG) is an extremely efficient enzyme that can remove uracil from any uracil-containing base pairs including the A/U base pair uracil + double-stranded DNA with abasic site
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 ?
uracil-mismatched single-stranded DNA + H2O Escherichia coli family 2 mismatch-specific uracil DNA glycosylase (MUG) is known to exhibit glycosylase activity on three mismatched base pairs, T/U, G/U and C/U. Family 1 uracil N-glycosylase (UNG) is an extremely efficient enzyme that can remove uracil from any uracil-containing base pairs including the A/U base pair uracil + single-stranded DNA with abasic site
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 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P12295
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-

Purification (Commentary)

Purification (Comment) Organism
HiTrap chelating column chromatography and HiTrap SP column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
uracil-mismatched double-stranded DNA + H2O family 2 mismatch-specific uracil DNA glycosylase (MUG) is known to exhibit glycosylase activity on three mismatched base pairs, T/U, G/U and C/U. Family 1 uracil N-glycosylase (UNG) is an extremely efficient enzyme that can remove uracil from any uracil-containing base pairs including the A/U base pair Escherichia coli uracil + double-stranded DNA with abasic site
-
 ?
uracil-mismatched single-stranded DNA + H2O family 2 mismatch-specific uracil DNA glycosylase (MUG) is known to exhibit glycosylase activity on three mismatched base pairs, T/U, G/U and C/U. Family 1 uracil N-glycosylase (UNG) is an extremely efficient enzyme that can remove uracil from any uracil-containing base pairs including the A/U base pair Escherichia coli uracil + single-stranded DNA with abasic site
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 ?

Synonyms

Synonyms Comment Organism
family 1 uracil N-glycosylase
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 Escherichia coli
UDG
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 Escherichia coli
UNG
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 Escherichia coli