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Literature summary for 3.2.2.27 extracted from
- Crystallization and preliminary X-ray diffraction analysis of three recombinant mutants of Vaccinia virus uracil DNA glycosylase (2013), Acta Crystallogr. Sect. F, 69, 295-301.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta(DE3)pLysS cells | Vaccinia virus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| mutant enzymes R167A, E171G/S172G/P173G, and DELTAE171/DELTAS172, hanging drop vapor diffusion method, using of 0.6-1.0 M ammonium sulfate, 0.1 M HEPES buffer pH 7.4, 10% (v/v) glycerol or 16% (w/v) PEG 3350, 0.15 M potassium fluoride, 10% (v/v) glycerol, or 2.0 M lithium sulfate in 50 mM MES buffer pH 5.6, 5 mM magnesium chloride, 5% (v/v) ethylene glycol | Vaccinia virus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25068 | - |
1 * 25068, calculated from amino acid sequence | Vaccinia virus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil-mismatched single-stranded DNA + H2O | Vaccinia virus | - |
uracil + single-stranded DNA with abasic site | - |
? |  |
| uracil-mismatched single-stranded DNA + H2O | Vaccinia virus Ankara | - |
uracil + single-stranded DNA with abasic site | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vaccinia virus | Q91UM2 | - |
- |
| Vaccinia virus Ankara | Q91UM2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| TALON column chromatography and Superdex 75 gel filtration | Vaccinia virus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| uracil-mismatched single-stranded DNA + H2O | - |
Vaccinia virus | uracil + single-stranded DNA with abasic site | - |
? | |
| uracil-mismatched single-stranded DNA + H2O | - |
Vaccinia virus Ankara | uracil + single-stranded DNA with abasic site | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 25068, calculated from amino acid sequence | Vaccinia virus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDG | - |
Vaccinia virus |
| uracil DNA glycosylase | - |
Vaccinia virus |
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