Literature summary for 3.2.2.27 extracted from

Chung, J.H.; Im, E.K.; Park, H.Y.; Kwon, J.H.; Lee, S.; Oh, J.; Hwang, K.C.; Lee, J.H.; Jang, Y.
A novel uracil-DNA glycosylase family related to the helix-hairpin-helix DNA glycosylase superfamily (2003), Nucleic Acids Res., 31, 2045-2055.

Search for more literature for 3.2.2.27

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron-sulfur cluster	the [4Fe-4S]-binding cluster is important for the DNA binding and catalytic activity	Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
24000	-	x * 24000, SDS-PAGE	Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
uracil-mismatched double-stranded DNA + H2O	Methanocaldococcus jannaschii	cytosine bases can be deaminated spontaneously to uracil, causing DNA damage. Uracil-DNA glycosylase repairs this kind of DNA damage	uracil + double-stranded DNA with abasic site	-	?
uracil-mismatched double-stranded DNA + H2O	Methanocaldococcus jannaschii DSM 2661	cytosine bases can be deaminated spontaneously to uracil, causing DNA damage. Uracil-DNA glycosylase repairs this kind of DNA damage	uracil + double-stranded DNA with abasic site	-	?

Organism

Organism	UniProt	Comment	Textmining
Methanocaldococcus jannaschii	Q58829	-	-
Methanocaldococcus jannaschii DSM 2661	Q58829	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
8-oxoguanine-mismatched double-stranded DNA + H2O	the enzyme effciently removes uracil from both single- and double-stranded DNA. Activity with oligonucleotide duplexes containing 8-oxoguanine is 5fold lower than that of oligonucleotide duplex containing U:T	Methanocaldococcus jannaschii	8-oxoguanine + double-stranded DNA with abasic site	-	?
8-oxoguanine-mismatched double-stranded DNA + H2O	the enzyme effciently removes uracil from both single- and double-stranded DNA. Activity with oligonucleotide duplexes containing 8-oxoguanine is 5fold lower than that of oligonucleotide duplex containing U:T	Methanocaldococcus jannaschii DSM 2661	8-oxoguanine + double-stranded DNA with abasic site	-	?
8-oxoguanine-mismatched single-stranded DNA + H2O	the enzyme effciently removes uracil from both single- and double-stranded DNA	Methanocaldococcus jannaschii	8-oxoguanine + single-stranded DNA with abasic site	-	?
8-oxoguanine-mismatched single-stranded DNA + H2O	the enzyme effciently removes uracil from both single- and double-stranded DNA	Methanocaldococcus jannaschii DSM 2661	8-oxoguanine + single-stranded DNA with abasic site	-	?
uracil-mismatched double-stranded DNA + H2O	cytosine bases can be deaminated spontaneously to uracil, causing DNA damage. Uracil-DNA glycosylase repairs this kind of DNA damage	Methanocaldococcus jannaschii	uracil + double-stranded DNA with abasic site	-	?
uracil-mismatched double-stranded DNA + H2O	the enzyme effciently removes uracil from both single- and double-stranded DNA. Insignificant differences in activity level on the mismatched duplex substrates U:T, U:C, U:G and U:A. oligonucleotide substrates containing 3-mA, 7-mG and an apurinic/apyrimidinic site are not hydrolyzed by the enzyme	Methanocaldococcus jannaschii	uracil + double-stranded DNA with abasic site	-	?
uracil-mismatched double-stranded DNA + H2O	cytosine bases can be deaminated spontaneously to uracil, causing DNA damage. Uracil-DNA glycosylase repairs this kind of DNA damage	Methanocaldococcus jannaschii DSM 2661	uracil + double-stranded DNA with abasic site	-	?
uracil-mismatched double-stranded DNA + H2O	the enzyme effciently removes uracil from both single- and double-stranded DNA. Insignificant differences in activity level on the mismatched duplex substrates U:T, U:C, U:G and U:A. oligonucleotide substrates containing 3-mA, 7-mG and an apurinic/apyrimidinic site are not hydrolyzed by the enzyme	Methanocaldococcus jannaschii DSM 2661	uracil + double-stranded DNA with abasic site	-	?
uracil-mismatched single-stranded DNA + H2O	the enzyme effciently removes uracil from both single- and double-stranded DNA	Methanocaldococcus jannaschii	uracil + single-stranded DNA with abasic site	-	?
uracil-mismatched single-stranded DNA + H2O	the enzyme effciently removes uracil from both single- and double-stranded DNA	Methanocaldococcus jannaschii DSM 2661	uracil + single-stranded DNA with abasic site	-	?

Subunits

Subunits	Comment	Organism
?	x * 24000, SDS-PAGE	Methanocaldococcus jannaschii

Synonyms

Synonyms	Comment	Organism
MjUDG	-	Methanocaldococcus jannaschii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	assay at	Methanocaldococcus jannaschii
55	65	-	Methanocaldococcus jannaschii

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
37	65	the enzyme is more active at 65°C than at 37°C	Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
80	-	30 min, stable	Methanocaldococcus jannaschii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	-	Methanocaldococcus jannaschii

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Release 2023.1 (January 2023)