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Literature summary for 3.2.2.27 extracted from

  • Begum, N.A.; Stanlie, A.; Doi, T.; Sasaki, Y.; Jin, H.W.; Kim, Y.S.; Nagaoka, H.; Honjo, T.
    Further evidence for involvement of a noncanonical function of uracil DNA glycosylase in class switch recombination (2009), Proc. Natl. Acad. Sci. USA, 106, 2752-2757.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
G87V a SMUG1 mutant, the mutation affects the thymine expulsion Mus musculus
G87Y a SMUG1 mutant, the mutation affects the thymine expulsion Mus musculus
H239L a SMUG1 mutant, the mutation affects the stabilization of transition state Mus musculus
additional information UNG deficiency reduces CSR efficiency to one tenth, but catalytically inactive mutants of UNG are fully proficient in CSR and several mutants at noncatalytic sites loose CSR activity. CSR activity by many UNG mutants critically depends on its N-terminal domain, irrespective of their enzymatic activities Mus musculus
N163D a SMUG1 mutant, the mutation affects the substrate binding Mus musculus
N85A a SMUG1 mutant, the mutation affects the H2O coordination Mus musculus

Inhibitors

Inhibitors Comment Organism Structure
Ugi a specific peptide inhibitor of UNG, inhibits class switch recombination without reducing DNA cleavage of the switch region, confirming dispensability of UNG in DNA cleavage in class switch recombination Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus deamination of cytosine in DNA leads to formation of uracil, which is removed by uracil DNA glycosylase, UNG. The N-terminus of UNG is required for class switch recombination activity, overview ?
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?

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information deamination of cytosine in DNA leads to formation of uracil, which is removed by uracil DNA glycosylase, UNG. The N-terminus of UNG is required for class switch recombination activity, overview Mus musculus ?
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?
additional information uracil DNA glycosylase uses single-stranded DNA substrate, a 5'-FITC-labeled oligonucleotide of 30mer with an internal single U residue Mus musculus ?
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?

Synonyms

Synonyms Comment Organism
SMUG1
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Mus musculus
UNG
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Mus musculus
uracil DNA glycosylase
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Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Mus musculus

General Information

General Information Comment Organism
physiological function involvement but dispensability of UNG in DNA cleavage in class switch recombination Mus musculus