Protein Variants | Comment | Organism |
---|---|---|
G87V | a SMUG1 mutant, the mutation affects the thymine expulsion | Mus musculus |
G87Y | a SMUG1 mutant, the mutation affects the thymine expulsion | Mus musculus |
H239L | a SMUG1 mutant, the mutation affects the stabilization of transition state | Mus musculus |
additional information | UNG deficiency reduces CSR efficiency to one tenth, but catalytically inactive mutants of UNG are fully proficient in CSR and several mutants at noncatalytic sites loose CSR activity. CSR activity by many UNG mutants critically depends on its N-terminal domain, irrespective of their enzymatic activities | Mus musculus |
N163D | a SMUG1 mutant, the mutation affects the substrate binding | Mus musculus |
N85A | a SMUG1 mutant, the mutation affects the H2O coordination | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ugi | a specific peptide inhibitor of UNG, inhibits class switch recombination without reducing DNA cleavage of the switch region, confirming dispensability of UNG in DNA cleavage in class switch recombination | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | deamination of cytosine in DNA leads to formation of uracil, which is removed by uracil DNA glycosylase, UNG. The N-terminus of UNG is required for class switch recombination activity, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | deamination of cytosine in DNA leads to formation of uracil, which is removed by uracil DNA glycosylase, UNG. The N-terminus of UNG is required for class switch recombination activity, overview | Mus musculus | ? | - |
? | |
additional information | uracil DNA glycosylase uses single-stranded DNA substrate, a 5'-FITC-labeled oligonucleotide of 30mer with an internal single U residue | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SMUG1 | - |
Mus musculus |
UNG | - |
Mus musculus |
uracil DNA glycosylase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
General Information | Comment | Organism |
---|---|---|
physiological function | involvement but dispensability of UNG in DNA cleavage in class switch recombination | Mus musculus |