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Literature summary for 3.2.2.27 extracted from
- Arabidopsis uracil DNA glycosylase (UNG) is required for base excision repair of uracil and increases plant sensitivity to 5-fluorouracil (2010), J. Biol. Chem., 285, 7475-7483.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant His-tagged enzymes in Escherichia coli strain BL21 (DE3) ung-151 | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D173F | site-directed mutagenesis | Arabidopsis thaliana |
| D173R | site-directed mutagenesis | Arabidopsis thaliana |
| additional information | the Arabidopsis mutant line GK-440E07 harbors a T-DNA insertion in the AtUNG gene. AtUNG-deficient plants do not display any apparent phenotype, but show increased resistance to 5-fluorouracil. The resistance of atung-/- mutants to 5-FU is accompanied by the accumulation of uracil residues in DNA | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Arabidopsis thaliana | 5634 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Arabidopsis thaliana | AtUNG is the major UDG activity in Arabidopsis thaliana AtUNG excises uracil in vivo but generates toxic AP sites when processing abundant U:A pairs in dTTP-depleted cells | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant His-tagged enzymes from Escherichia coli strain BL21 (DE3) ung-151 by nickel affinity chromatography | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | AtUNG is the major UDG activity in Arabidopsis thaliana AtUNG excises uracil in vivo but generates toxic AP sites when processing abundant U:A pairs in dTTP-depleted cells | Arabidopsis thaliana | ? | - |
? | |
| additional information | AtUNG exhibits the narrow substrate specificity and single-stranded DNA preference, it is active on 5'-labeled 51mer oligonucleotide duplex containing a U:G mispair. AtUNG is significantly more active on U:G mispairs than on U:A pairs. The activity on U:T and U:C mispairs is slightly lower than on U:G, but higher than on U:A pairs | Arabidopsis thaliana | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | AtUng is a plant member of the family-1 of UDGs typified by Escherichia coli Ung | Arabidopsis thaliana |
| UNG | - |
Arabidopsis thaliana |
| uracil DNA glycosylase | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 37 | assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | assay at | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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