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Literature summary for 3.2.2.27 extracted from
- Loss of Caenorhabditis elegans UNG-1 uracil-DNA glycosylase affects apoptosis in response to DNA damaging agents (2010), DNA Repair, 9, 861-870.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ung-1, expression of the N-terminally GST-tagged enzyme in Escherichia coli strain BL21 ung151 (ung::Tn10(Tet)) | Caenorhabditis elegans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | an ung-1 mutant has reduced ability to repair uracil-containing DNA. Ung-1 mutants show altered levels of apoptotic cell corpses formed in response to DNA damaging agents and increased apoptosis in response to ionizing radiation. The phenotype is a consequence of compensatory transcriptomic shifts that modulate oxidative stress responses in the mutant and not an effect of reduced DNA damage signaling, overview. Attenuation of paraquat-induced apoptosis in ung-1 mutant results from modulation of stress-induced signaling pathways, overview | Caenorhabditis elegans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ugi | a peptide inhibitor specific for UNG-family enzyme | Caenorhabditis elegans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
x * 60000, recombinant N-terminally GST-tagged enzyme, SDS-PAGE, x * 32000, detagged enzyme, SDS-PAGE | Caenorhabditis elegans |
| 60000 | - |
x * 60000, recombinant N-terminally GST-tagged enzyme, SDS-PAGE, x * 32000, detagged enzyme, SDS-PAGE | Caenorhabditis elegans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caenorhabditis elegans | - |
gene ung-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally GST-tagged enzyme from Escherichia coli strain BL21 ung151 (ung::Tn10(Tet)) by glutathione affinity chromatography, cleavage of the tag by thrombin and further purification by cationic exchange chromatography | Caenorhabditis elegans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme shows activity on double-stranded as well as single-stranded DNAs, the assay uses a 19mer oligodeoxyribonucleotide substrate containing a single uracil residue (5'-CATAAAGTGUAAAGCCTGG-3') and a guanine or adenine residue opposite uracil | Caenorhabditis elegans | ? | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, recombinant N-terminally GST-tagged enzyme, SDS-PAGE, x * 32000, detagged enzyme, SDS-PAGE | Caenorhabditis elegans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the enzyme belongs to the UNG family of enzymes | Caenorhabditis elegans |
| UDG | - |
Caenorhabditis elegans |
| UNG-1 | - |
Caenorhabditis elegans |
| uracil-DNA glycosylase | - |
Caenorhabditis elegans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Caenorhabditis elegans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Caenorhabditis elegans |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | UNG-1 contributes to repair of ionizing radiation-induced DNA base damage in vivo | Caenorhabditis elegans |
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Release 2023.1 (January 2023)
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