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Literature summary for 3.2.2.27 extracted from
- The oxidative DNA glycosylases of Mycobacterium tuberculosis exhibit different substrate preferences from their Escherichia coli counterparts (2010), DNA Repair, 9, 177-190.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the interaction between Lhr and MtuNei2 may be crucial for recruiting MtuNei2 onto damaged DNA and stimulating the activity of MtuNei2 during DNA repair | Mycobacterium tuberculosis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| genes Rv2464c and Rv3297, expression of His-tagged enzymes from a bicistronic vector in Escherichia coli | Mycobacterium tuberculosis |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | nei nth double mutants exhibit an elevated spontaneous mutation frequency. Spontaneous mutations observed in the double mutant are solely from C to T transitions due to the inability to repair oxidized cytosines, which can be efficiently prevented by MtuNei1 and MtuNei2 | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of MtuNei1 with diverse substrates, overview | Mycobacterium tuberculosis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28500 | - |
x * 28500, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Mycobacterium tuberculosis | MtuNei1 recognizes oxidized pyrimidines on both double-stranded and single-stranded DNA and exhibits uracil DNA glycosylase activity. MtuNei2 and MtuNei1 can prevent G to T transversions probably by removing oxidized guanine products, such as Sp and urea | ? | - |
? |  |
| additional information | Mycobacterium tuberculosis H37Rv | MtuNei1 recognizes oxidized pyrimidines on both double-stranded and single-stranded DNA and exhibits uracil DNA glycosylase activity. MtuNei2 and MtuNei1 can prevent G to T transversions probably by removing oxidized guanine products, such as Sp and urea | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
genes Rv2464c and Rv3297, encoding MtuNei1 and MtuNei2 | - |
| Mycobacterium tuberculosis H37Rv | - |
genes Rv2464c and Rv3297, encoding MtuNei1 and MtuNei2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzymes from Escherichia coli by nickel affinity chromatograpyh | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | MtuNei1 recognizes oxidized pyrimidines on both double-stranded and single-stranded DNA and exhibits uracil DNA glycosylase activity. MtuNei2 and MtuNei1 can prevent G to T transversions probably by removing oxidized guanine products, such as Sp and urea | Mycobacterium tuberculosis | ? | - |
? | |
| additional information | MtuNei1 recognizes oxidized pyrimidines on both double-stranded and single-stranded DNA and exhibits uracil DNA glycosylase activity. MtuNei1 excises thymine glycol and strongly prefers the 5R isomers. GC/MS analysis of products released by MtuNei1, overview. Substrates are oligonucleotides with 7,8-dihydro-8-oxoguanine, thymine glycol, 5,6-dihydrouracil, 5,6-dihydrothymine, 5-hydroxyuracil, 5-hydroxycytosine, uracil, and furan | Mycobacterium tuberculosis | ? | - |
? | |
| additional information | MtuNei1 recognizes oxidized pyrimidines on both double-stranded and single-stranded DNA and exhibits uracil DNA glycosylase activity. MtuNei2 and MtuNei1 can prevent G to T transversions probably by removing oxidized guanine products, such as Sp and urea | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
| additional information | MtuNei1 recognizes oxidized pyrimidines on both double-stranded and single-stranded DNA and exhibits uracil DNA glycosylase activity. MtuNei1 excises thymine glycol and strongly prefers the 5R isomers. GC/MS analysis of products released by MtuNei1, overview. Substrates are oligonucleotides with 7,8-dihydro-8-oxoguanine, thymine glycol, 5,6-dihydrouracil, 5,6-dihydrothymine, 5-hydroxyuracil, 5-hydroxycytosine, uracil, and furan | Mycobacterium tuberculosis H37Rv | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 28500, recombinant enzyme, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endonuclease VIII | - |
Mycobacterium tuberculosis |
| MtuNei1 | - |
Mycobacterium tuberculosis |
| UDG | - |
Mycobacterium tuberculosis |
| uracil DNA glycosylase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Mycobacterium tuberculosis |
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