Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant UDGs in Escherichia coli strain BL21(DE3) | Chlamydia pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
A205S | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Chlamydia pneumoniae |
D77N | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Chlamydia pneumoniae |
H200Q | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Chlamydia pneumoniae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | free uracil and abasic sites inhibit the enzyme reaction | Chlamydia pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0052 | - |
uracil-mismatched double-stranded DNA | pH 8.0, 37°C, recombinant wild-type enzyme | Chlamydia pneumoniae | |
0.009 | - |
uracil-mismatched double-stranded DNA | pH 8.0, 37°C, recombinant mutant A205S | Chlamydia pneumoniae | |
0.148 | - |
uracil-mismatched double-stranded DNA | pH 8.0, 37°C, recombinant mutant H200Q | Chlamydia pneumoniae | |
0.256 | - |
uracil-mismatched double-stranded DNA | pH 8.0, 37°C, recombinant mutant D77N | Chlamydia pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydia pneumoniae | Q9Z7D3 | - |
- |
Chlamydia pneumoniae AR39 | Q9Z7D3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant UDGs from Escherichia coli strain BL21 (DE3) by nickel affinity and anion exchange chromatography | Chlamydia pneumoniae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
purified recombinant enzyme | Chlamydia pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil-mismatched double-stranded DNA + H2O | duplex DNA with A/U mismatch, substrate specificity, the base opposite to uracil in double strand DNAs affects uracil removal efficiencies in descending order U/-, U/T, U/C, U/G, U/A. Free uracil and abasic sites inhibit the reaction. Amino acids D77, H200, and A205 are important for the catalytic activity of UDG | Chlamydia pneumoniae | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | duplex DNA with A/U mismatch, substrate specificity, the base opposite to uracil in double strand DNAs affects uracil removal efficiencies in descending order U/-, U/T, U/C, U/G, U/A. Free uracil and abasic sites inhibit the reaction. Amino acids D77, H200, and A205 are important for the catalytic activity of UDG | Chlamydia pneumoniae AR39 | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA with A-U mismatch + H2O | - |
Chlamydia pneumoniae | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA with A-U mismatch + H2O | - |
Chlamydia pneumoniae AR39 | uracil + double-stranded DNA with abasic site | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | the enzyme is a member of the UDG family I | Chlamydia pneumoniae |
UDG | - |
Chlamydia pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Chlamydia pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
uracil-mismatched double-stranded DNA | pH 8.0, 37°C, recombinant mutant D77N | Chlamydia pneumoniae | |
0.053 | - |
uracil-mismatched double-stranded DNA | pH 8.0, 37°C, recombinant mutant H200Q | Chlamydia pneumoniae | |
0.73 | - |
uracil-mismatched double-stranded DNA | pH 8.0, 37°C, recombinant mutant A205S | Chlamydia pneumoniae | |
5.9 | - |
uracil-mismatched double-stranded DNA with A-U mismatch + | pH 8.0, 37°C, recombinant wild-type enzyme | Chlamydia pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Chlamydia pneumoniae |