Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.27 extracted from

  • Bellamy, S.R.; Baldwin, G.S.
    A kinetic analysis of substrate recognition by uracil-DNA glycosylase from Herpes simplex virus type I (2001), Nucleic Acids Res., 29, 3857-3863.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli strain BL834 Human alphaherpesvirus 1

Protein Variants

Protein Variants Comment Organism
D88N site-directed mutagenesis of the active site Asp88, catalytically inactive mutant, analysis of substrate binding , overview Human alphaherpesvirus 1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics with several oligonucleitide substrate possessing U-A or U-G mispairs, overview Human alphaherpesvirus 1

Organism

Organism UniProt Comment Textmining
Human alphaherpesvirus 1
-
HSV-1
-
Human alphaherpesvirus 1 HSV-1
-
HSV-1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli strain BL834 by two steps of ion exchange chromatography Human alphaherpesvirus 1

Reaction

Reaction Comment Organism Reaction ID
Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil the active site catalytic residue is Asp88 Human alphaherpesvirus 1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information UDG is responsible for the removal of uracil from DNA with U-A or U-G mismatch, UDG is active on single- or double-stranded DNA, the damage recognition step in the HSV-1 UDG reaction pathway is modulated by local DNA sequences, substrate specificity with several oligonucleotide substrate possessing U-A or U-G mispairs, overview Human alphaherpesvirus 1 ?
-
?
additional information UDG is responsible for the removal of uracil from DNA with U-A or U-G mismatch, UDG is active on single- or double-stranded DNA, the damage recognition step in the HSV-1 UDG reaction pathway is modulated by local DNA sequences, substrate specificity with several oligonucleotide substrate possessing U-A or U-G mispairs, overview Human alphaherpesvirus 1 HSV-1 ?
-
?
uracil-mismatched double-stranded DNA + H2O
-
Human alphaherpesvirus 1 uracil + double-stranded DNA with abasic site
-
?
uracil-mismatched double-stranded DNA + H2O
-
Human alphaherpesvirus 1 HSV-1 uracil + double-stranded DNA with abasic site
-
?
uracil-mismatched single-stranded DNA + H2O
-
Human alphaherpesvirus 1 uracil + single-stranded DNA with abasic site
-
?
uracil-mismatched single-stranded DNA + H2O
-
Human alphaherpesvirus 1 HSV-1 uracil + single-stranded DNA with abasic site
-
?

Synonyms

Synonyms Comment Organism
UDG
-
Human alphaherpesvirus 1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Human alphaherpesvirus 1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Human alphaherpesvirus 1