Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli strain BL834 | Human alphaherpesvirus 1 |
Protein Variants | Comment | Organism |
---|---|---|
D88N | site-directed mutagenesis of the active site Asp88, catalytically inactive mutant, analysis of substrate binding , overview | Human alphaherpesvirus 1 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics with several oligonucleitide substrate possessing U-A or U-G mispairs, overview | Human alphaherpesvirus 1 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Human alphaherpesvirus 1 | - |
HSV-1 | - |
Human alphaherpesvirus 1 HSV-1 | - |
HSV-1 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL834 by two steps of ion exchange chromatography | Human alphaherpesvirus 1 |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil | the active site catalytic residue is Asp88 | Human alphaherpesvirus 1 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | UDG is responsible for the removal of uracil from DNA with U-A or U-G mismatch, UDG is active on single- or double-stranded DNA, the damage recognition step in the HSV-1 UDG reaction pathway is modulated by local DNA sequences, substrate specificity with several oligonucleotide substrate possessing U-A or U-G mispairs, overview | Human alphaherpesvirus 1 | ? | - |
? | |
additional information | UDG is responsible for the removal of uracil from DNA with U-A or U-G mismatch, UDG is active on single- or double-stranded DNA, the damage recognition step in the HSV-1 UDG reaction pathway is modulated by local DNA sequences, substrate specificity with several oligonucleotide substrate possessing U-A or U-G mispairs, overview | Human alphaherpesvirus 1 HSV-1 | ? | - |
? | |
uracil-mismatched double-stranded DNA + H2O | - |
Human alphaherpesvirus 1 | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | - |
Human alphaherpesvirus 1 HSV-1 | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | - |
Human alphaherpesvirus 1 | uracil + single-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | - |
Human alphaherpesvirus 1 HSV-1 | uracil + single-stranded DNA with abasic site | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDG | - |
Human alphaherpesvirus 1 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Human alphaherpesvirus 1 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Human alphaherpesvirus 1 |