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Literature summary for 3.2.2.27 extracted from

  • Priet, S.; Gros, N.; Navarro, J.-M.; Boretto, J.I.; Canard, B.; Querat, G.; Sire, J.
    HIV-1-associated uracil DNA glycosylase activity controls dUTP misincorporation in viral DNA and is essential to the HIV-1 life cycle (2005), Mol. Cell, 17, 479-490.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information depletion of UNG2 in macrophages by siRNA, HIV-1 virus fails to replicate in UNG2-depleted macrophages, UNG1 cannot compensate. Depletion of UNG2 in producer MAGI-CCR5 cells generates noninfectious virus, overview. Restoration of viral infectivity of UNG2-deficient virus by transfection of dUTPase-expressing vector in UNG2-depleted producer cells Homo sapiens
Q152L/D154E a siRNA-insensitive, inactive UNG2 mutant, overexpression in UNG2-depleted MAGI-CCR5 producer cells fails to restore viral infectivity Homo sapiens
W231A/F234G site-directed mutagenesis, the mutation impairs the association of UNG2 with viral protein Vpr UNG2-depleted MAGI-CCR5 producer cells and viral infectivity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Ugi peptide
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Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens uracil DNA glycosylase acts in removing uracil from the sugar backbone of DNA, leaving abasic sites and initiating the uracil base-excision-repair pathway, BER. The human UNG2 enzyme, but not UNG1, is packaged and incorporated into HIV-1 virions via specific interaction with the integrase domain of the Gag-Pol precursor, the virally Vpr protein might also able to mediate the incorporation of UNG2, packaged UNG2 can process uracil from DNA, indicating that HIV-1 has the ability to control dUTP misincorporation in viral DNA, the enzyme is essential to the HIV-1 life cycle. HIV-1 RT and UNG2 recombinant proteins can process uracil from primer-template substrate, molecular mechanism ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P22674 UNG2
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Source Tissue

Source Tissue Comment Organism Textmining
HeLa-MAGI-CCR5 cell
-
Homo sapiens
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macrophage
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information uracil DNA glycosylase acts in removing uracil from the sugar backbone of DNA, leaving abasic sites and initiating the uracil base-excision-repair pathway, BER. The human UNG2 enzyme, but not UNG1, is packaged and incorporated into HIV-1 virions via specific interaction with the integrase domain of the Gag-Pol precursor, the virally Vpr protein might also able to mediate the incorporation of UNG2, packaged UNG2 can process uracil from DNA, indicating that HIV-1 has the ability to control dUTP misincorporation in viral DNA, the enzyme is essential to the HIV-1 life cycle. HIV-1 RT and UNG2 recombinant proteins can process uracil from primer-template substrate, molecular mechanism Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
UNG2
-
Homo sapiens
uracil DNA glycosylase
-
Homo sapiens