Cloned (Comment) | Organism |
---|---|
SMUG1 is encoded within a small region of human chromosome 12, cloning from HeLa S3 cells, expression of GST-tagged SMUG1 in Escherichia coli strain BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition of SMUG1 by uracil glycosylase inhibitor | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | SMUG1 single-turnover kinetics using oligonucleotide substrates containing purine and pyrimidine analogs | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | hSMUG1 removes uracil from both double- and single-stranded DNA, including 5-hydroxy-2'-deoxyuridine and 5-carboxy-2'-deoxyuridine, substrate selectivity mechanism, overview | ? | - |
? | |
uracil-mismatched double-stranded DNA + H2O | Homo sapiens | - |
uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | Homo sapiens | - |
uracil + single-stranded DNA with abasic site | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q53HV7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged SMUG1 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, the tag is cleaved off by thrombin | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa-S3 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | hSMUG1 removes uracil from both double- and single-stranded DNA, including 5-hydroxy-2'-deoxyuridine and 5-carboxy-2'-deoxyuridine, substrate selectivity mechanism, overview | Homo sapiens | ? | - |
? | |
additional information | preference of hSMUG1 for mispaired uracil over uracil paired with adenine, substrate selectivity with oligonucleotide 24-mers containing uracil with different 5-substituents, overview | Homo sapiens | ? | - |
? | |
uracil-mismatched double-stranded DNA + H2O | - |
Homo sapiens | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | - |
Homo sapiens | uracil + single-stranded DNA with abasic site | - |
? |
Synonyms | Comment | Organism |
---|---|---|
single-strand selective monofunctional uracil-DNA glycosylase | - |
Homo sapiens |
SMUG1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Homo sapiens |