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Literature summary for 3.2.2.27 extracted from

  • Haushalter, K.A
    Stukenberg, P.T.; Kirschner, M.W.; Verdine, G.L.: Identification of a new uracil-DNA glycosylase family by expression cloning using synthetic inhibitors (1999), Curr. Biol., 9, 174-185.
    View publication on PubMed
Search for more literature for 3.2.2.27

Cloned(Commentary)

Cloned (Comment) Organism
clone TD12, DNA and amino acid sequence determination and analysis, expression as C-terminally His6-tagged enzyme in Escherichia coli Xenopus laevis
DNA and amino acid sequence determination and analysis Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information design of mechanism-based inhibitors, overview Homo sapiens
additional information design of mechanism-based inhibitors, overview Xenopus laevis
UGI inhibitor
-
 Homo sapiens
UGI inhibitor
-
 Xenopus laevis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics Xenopus laevis
0.000035
-
 uracil-mismatched double-stranded DNA with U-G mismatch
-
 Xenopus laevis
0.00109
-
 uracil-mismatched single-stranded DNA
-
 Xenopus laevis

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Xenopus laevis 5634
-
nucleus
-
 Homo sapiens 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
 x * 31000, SMUG1, SDS-PAGE Xenopus laevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uracil-mismatched double-stranded DNA + H2O Xenopus laevis
-
 uracil + double-stranded DNA with abasic site
-
 ?
uracil-mismatched single-stranded DNA + H2O Xenopus laevis
-
 uracil + single-stranded DNA with abasic site
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q53HV7
-
-
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His6-tagged enzyme from Escherichia coli by a 2-step procedure Xenopus laevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information SMUG1 is a monofunctional DNA glycosylase specific for uracil residues, and has appreciable selectivity for single-stranded rather than double-stranded DNA substrates, overview Xenopus laevis ?
-
 ?
additional information SMUG1 is a monofunctional DNA glycosylase specific for uracil residues, and has appreciable selectivity for single-stranded rather than double-stranded DNA substrates, overview Homo sapiens ?
-
 ?
uracil-mismatched double-stranded DNA + H2O
-
 Xenopus laevis uracil + double-stranded DNA with abasic site
-
 ?
uracil-mismatched double-stranded DNA + H2O
-
 Homo sapiens uracil + double-stranded DNA with abasic site
-
 ?
uracil-mismatched double-stranded DNA with U-G mismatch + H2O
-
 Xenopus laevis uracil + double-stranded DNA with abasic site
-
 ?
uracil-mismatched single-stranded DNA + H2O
-
 Xenopus laevis uracil + single-stranded DNA with abasic site
-
 ?
uracil-mismatched single-stranded DNA + H2O
-
 Homo sapiens uracil + single-stranded DNA with abasic site
-
 ?

Subunits

Subunits Comment Organism
? x * 31000, SMUG1, SDS-PAGE Xenopus laevis

Synonyms

Synonyms Comment Organism
single-strand-selective monofunctional uracil-DNA glycosylase
-
 Xenopus laevis
single-strand-selective monofunctional uracil-DNA glycosylase
-
 Homo sapiens
SMUG1
-
 Xenopus laevis
SMUG1
-
 Homo sapiens
TD12-encoded protein
-
 Xenopus laevis
uracil-DNA glycosylase
-
 Xenopus laevis
uracil-DNA glycosylase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0014
-
 uracil-mismatched double-stranded DNA with U-G mismatch
-
 Xenopus laevis
2.5
-
 uracil-mismatched single-stranded DNA
-
 Xenopus laevis