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Literature summary for 3.2.2.27 extracted from
- Characterization of heat-labile uracil-DNA glycosylase from Psychrobacter sp. HJ147 and its application to the polymerase chain reaction (2009), Biotechnol. Appl. Biochem., 52, 167-175.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | UDG forms an apyrimidinic site, where an uracil base is removed, and facilitates the reactions of various DNA-repairing enzymes, such as AP endonuclease, DNA polymerase, and DNA ligase, it is thus useful in applications to remove uracil-DNA. Use of UDG to control carry-over contamination in PCR | Psychrobacter sp. |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Psp HJ147 UDG gene, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression of UDG containing an N-terminal intein cleavage site with an affinity chitin-binding tag under the control of a T7lac promoter, pTYB1 plasmid, in Escherichia coli strain BL21(DE3) | Psychrobacter sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Psychrobacter sp. | D3G6S3 | - |
- |
| Psychrobacter sp. HJ147 | D3G6S3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant UDG from Escherichia coli strain BL21(DE3) by chitin affinity chromatogaphy to homogeneity, the tag is cleaved off by intein | Psychrobacter sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | UDG specifically and selectively removes uracil bases from DNA, substrate specificity, overview | Psychrobacter sp. | ? | - |
? |  |
| additional information | UDG specifically and selectively removes uracil bases from DNA, substrate specificity, overview | Psychrobacter sp. HJ147 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDG | - |
Psychrobacter sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
recombinant UDG | Psychrobacter sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 50 | recombinant UDG, inactive above 50°C, temperature profile | Psychrobacter sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
heat-labile enzyme | Psychrobacter sp. |
| 30 | - |
rapid decrease of enzyme activity above 30°C | Psychrobacter sp. |
| 40 | - |
purified recombinant enzyme, half-life is 2 min | Psychrobacter sp. |
| 50 | - |
purified recombinant enzyme, half-life is 45 s | Psychrobacter sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | recombinant UDG | Psychrobacter sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | recombinant UDG, inactive at pH 5.5, pH profile | Psychrobacter sp. |
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