Cloned (Comment) | Organism |
---|---|
SMUG1 from kidney, expression of GST-tagged SMUG1 in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
SMUG1 from liver cDNA library, expression of GST-tagged SMUG1 in Escherichia coli strain BL21(DE3) | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-formyluracil-mismatched double-stranded DNA + H2O | Homo sapiens | - |
5-formyluracil + double-stranded DNA with abasic site | - |
? | |
5-formyluracil-mismatched single-stranded DNA + H2O | Homo sapiens | - |
5-formyluracil + single-stranded DNA with abasic site | - |
? | |
5-hydroxymethyluracil-mismatched single-stranded DNA + H2O | Homo sapiens | - |
5-hydroxymethyluracil + single-stranded DNA with abasic site | - |
? | |
5-hydroxymethyluracil-mismatched single-stranded DNA + H2O | Rattus norvegicus | - |
5-hydroxymethyluracil + single-stranded DNA with abasic site | - |
? | |
5-hydroxyuracil-mismatched single-stranded DNA + H2O | Homo sapiens | - |
5-hydroxyuracil + single-stranded DNA with abasic site | - |
? | |
5-hydroxyuracil-mismatched single-stranded DNA + H2O | Rattus norvegicus | - |
5-hydroxyuracil + single-stranded DNA with abasic site | - |
? | |
additional information | Homo sapiens | dual role of hSMUG1 as a backup enzyme for UNG and a primary repair enzyme for a subset of oxidized pyrimidines such as 5-formyluracil, 5-hydroxymethyluracil, and 5-hydroxyuracil | ? | - |
? | |
uracil-mismatched double-stranded DNA + H2O | Homo sapiens | - |
uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | Rattus norvegicus | - |
uracil + double-stranded DNA with abasic site | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q53HV7 | - |
- |
Rattus norvegicus | Q811Q1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged SMUG1 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, the tag is cleaved off by thrombin | Homo sapiens |
recombinant GST-tagged SMUG1 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, the tag is cleaved off by thrombin | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-formyluracil-mismatched double-stranded DNA + H2O | - |
Homo sapiens | 5-formyluracil + double-stranded DNA with abasic site | - |
? | |
5-formyluracil-mismatched single-stranded DNA + H2O | - |
Homo sapiens | 5-formyluracil + single-stranded DNA with abasic site | - |
? | |
5-hydroxymethyluracil-mismatched double-stranded DNA + H2O | - |
Homo sapiens | 5-hydroxymethyluracil + double-stranded DNA with abasic site | - |
? | |
5-hydroxymethyluracil-mismatched single-stranded DNA + H2O | - |
Homo sapiens | 5-hydroxymethyluracil + single-stranded DNA with abasic site | - |
? | |
5-hydroxymethyluracil-mismatched single-stranded DNA + H2O | - |
Rattus norvegicus | 5-hydroxymethyluracil + single-stranded DNA with abasic site | - |
? | |
5-hydroxyuracil-mismatched double-stranded DNA + H2O | - |
Homo sapiens | 5-hydroxyuracil + double-stranded DNA with abasic site | - |
? | |
5-hydroxyuracil-mismatched single-stranded DNA + H2O | - |
Homo sapiens | 5-hydroxyuracil + single-stranded DNA with abasic site | - |
? | |
5-hydroxyuracil-mismatched single-stranded DNA + H2O | - |
Rattus norvegicus | 5-hydroxyuracil + single-stranded DNA with abasic site | - |
? | |
additional information | substrate specificity, overview | Rattus norvegicus | ? | - |
? | |
additional information | dual role of hSMUG1 as a backup enzyme for UNG and a primary repair enzyme for a subset of oxidized pyrimidines such as 5-formyluracil, 5-hydroxymethyluracil, and 5-hydroxyuracil | Homo sapiens | ? | - |
? | |
additional information | substrate specificity, overview. hSMUG1 removes damaged bases from Fenton-oxidized calf thymus DNA, generating abasic sites | Homo sapiens | ? | - |
? | |
uracil-mismatched double-stranded DNA + H2O | - |
Homo sapiens | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | - |
Rattus norvegicus | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched single-stranded DNA + H2O | - |
Homo sapiens | uracil + single-stranded DNA with abasic site | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5-formyluracil-DNA glycosylase | - |
Homo sapiens |
5-formyluracil-DNA glycosylase | - |
Rattus norvegicus |
SMUG1 | - |
Homo sapiens |
SMUG1 | - |
Rattus norvegicus |
uracil-DNA glycosylase | - |
Homo sapiens |
uracil-DNA glycosylase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
7.5 | - |
assay at | Rattus norvegicus |