Cloned (Comment) | Organism |
---|---|
gene dr0689, expression of His-tagged UNG type 1 in Escherichia coli strain BL21(DE3) | Deinococcus radiodurans |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged UNG type 1, hanging drop vapour diffusion method, 0.001 ml of 10 mg/ml protein in 70 mM Tris-HCl, 10 mM NaCl, 1 mM EDTA pH 8.0, 100 mg/ml bovine serum albumin, are mixed with 0.001 ml of precipitant solution containing 0.2 M ammonium nitrate and 17% w/v PEG 3000 at 4°C for a few days, 20% v/v glycerol as cryoprotectant, X-ray diffraction structure determination and analysis at 1.8-1.9 A resolution | Deinococcus radiodurans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Deinococcus radiodurans | |
0.0007 | - |
uracil-mismatched double-stranded DNA | pH 8.0, 37°C, recombinant enzyme | Deinococcus radiodurans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27700 | - |
x * 27700, about, sequence calculation | Deinococcus radiodurans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil-mismatched double-stranded DNA + H2O | Deinococcus radiodurans | the enzyme removes uracil from DNA, which can occur by misincorporation of dUMP in place of dTMP during DNA synthesis or by deamination of cytosine, resulting in U-A or U-G mispairs | uracil + double-stranded DNA with abasic site | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deinococcus radiodurans | Q9RWH9 | gene dr0689 encoding the type 1 UDG, i.e. UNG | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged UNG type 1 from Escherichia coli strain BL21(DE3) by affinity chromatography | Deinococcus radiodurans |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Hydrolyses single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil | uracil-DNA glycosylases are widespread enzymes that are found in all living organisms. UDGs form a central part of the DNA-repair machinery since they initiate the DNA base-excision repair pathway by hydrolysing the N-glycosidic bond between uracil and the deoxyribose sugar thereby catalysing the removal of mis-incorporated uracil from DNA | Deinococcus radiodurans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil-mismatched double-stranded DNA + H2O | the enzyme removes uracil from DNA, which can occur by misincorporation of dUMP in place of dTMP during DNA synthesis or by deamination of cytosine, resulting in U-A or U-G mispairs | Deinococcus radiodurans | uracil + double-stranded DNA with abasic site | - |
? | |
uracil-mismatched double-stranded DNA + H2O | drUNG is able to excise uracil from both U-A and U-G double-stranded DNA and single-stranded DNA, substrate is commercially available nick-translated calf thymus DNA with deoxy[5-3H]uridine 5'-triphosphate: DNA binding, the active-site environment, and uracil specificity, structure-activity relationship, overview | Deinococcus radiodurans | uracil + double-stranded DNA with abasic site | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27700, about, sequence calculation | Deinococcus radiodurans |
More | type 1 UNG structure analysis and comparison with the human and Escherichia coli enzymes, structure-activity relationship, overview | Deinococcus radiodurans |
Synonyms | Comment | Organism |
---|---|---|
dr0689 | - |
Deinococcus radiodurans |
type 1 UDG | - |
Deinococcus radiodurans |
UDG | - |
Deinococcus radiodurans |
UNG | - |
Deinococcus radiodurans |
uracil-DNA N-glycosylase | - |
Deinococcus radiodurans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Deinococcus radiodurans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
15.58 | - |
uracil-mismatched double-stranded DNA | pH 8.0, 37°C, recombinant enzyme | Deinococcus radiodurans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Deinococcus radiodurans |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Deinococcus radiodurans | sequence calculation | - |
6.97 |