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Literature summary for 3.2.2.24 extracted from

  • Moure, V.R.; Sioeberg, C.L.B.; Valdameri, G.; Nji, E.; Oliveira, M.A.S.; Gerdhardt, E.C.M.; Pedrosa, F.O.; Mitchell, D.A.; Seefeldt, L.C.; Huergo, L.F.; Hoegbom, M.; Nordlund, S.; Souza, E.M.
    The ammonium transporter AmtB and the PII signal transduction protein GlnZ are required to inhibit DraG in Azospirillum brasilense (2019), FEBS J., 286, 1214-1229 .
    View publication on PubMed
Search for more literature for 3.2.2.24

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Azospirillum brasilense

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 35% (v/v) glycerol ethoxylate and 0.2 M lithium citrate Azospirillum brasilense

Inhibitors

Inhibitors Comment Organism Structure
GlnZ the enzyme is partially inhibited by GlnZ. Enzyme inactivation requires the formation of a ternary enzyme-GlnZ-AmtB complex in vitro Azospirillum brasilense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ADP-ribosylated Fe-protein Azospirillum brasilense
-
 ADP-D-ribose + Fe-protein
-
 ?
ADP-ribosylated Fe-protein Azospirillum brasilense FP2
-
 ADP-D-ribose + Fe-protein
-
 ?

Organism

Organism UniProt Comment Textmining
Azospirillum brasilense A7XNI2
-
-
Azospirillum brasilense FP2 A7XNI2
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap heparin column chromatography or His-Trap column chromatography Azospirillum brasilense

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-ribosylated Fe-protein
-
 Azospirillum brasilense ADP-D-ribose + Fe-protein
-
 ?
ADP-ribosylated Fe-protein
-
 Azospirillum brasilense FP2 ADP-D-ribose + Fe-protein
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Azospirillum brasilense

Synonyms

Synonyms Comment Organism
dinitrogenase reductase ADP-glycohydrolase
-
 Azospirillum brasilense
DRAG
-
 Azospirillum brasilense