Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.2.23 extracted from

  • Li, H.; Endutkin, A.V.; Bergonzo, C.; Campbell, A.J.; de los Santos, C.; Grollman, A.; Zharkov, D.O.; Simmerling, C.
    A dynamic checkpoint in oxidative lesion discrimination by formamidopyrimidine-DNA glycosylase (2016), Nucleic Acids Res., 44, 683-694 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.23

Protein Variants

Protein Variants Comment Organism
N168D 0.2% of wild-type activity Escherichia coli
N168Q 9.5% of wild-type activity Escherichia coli
R108K 1.9% of wild-type activity Escherichia coli
R108L 0.3% of wild-type activity Escherichia coli
R108Q 0.3% of wild-type activity Escherichia coli
R258A 0.4% of wild-type activity Escherichia coli
R258K 0.3% of wild-type activity Escherichia coli
R258Q 6.2% of wild-type activity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0000063
-
 23mer oligonucleotide duplex containing 8-oxoguanine mutant R258Q, pH 7.5, 30°C Escherichia coli
0.0000081
-
 23mer oligonucleotide duplex containing 8-oxoguanine wild-type, pH 7.5, 30°C Escherichia coli
0.0000081
-
 23mer oligonucleotide duplex containing 8-oxoguanine mutant N168Q, pH 7.5, 30°C Escherichia coli
0.000018
-
 23mer oligonucleotide duplex containing 8-oxoguanine mutant R108K, pH 7.5, 30°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P05523
-
-
Geobacillus stearothermophilus P84131
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
23mer oligonucleotide duplex containing 8-oxoguanine + H2O
-
 Escherichia coli 8-oxoguanine + oligonucleotide
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
 23mer oligonucleotide duplex containing 8-oxoguanine mutant R108K, pH 7.5, 30°C Escherichia coli
0.08
-
 23mer oligonucleotide duplex containing 8-oxoguanine mutant R258Q, pH 7.5, 30°C Escherichia coli
0.16
-
 23mer oligonucleotide duplex containing 8-oxoguanine mutant N168Q, pH 7.5, 30°C Escherichia coli
1.7
-
 23mer oligonucleotide duplex containing 8-oxoguanine wild-type, pH 7.5, 30°C Escherichia coli

General Information

General Information Comment Organism
physiological function Fpg selectively facilitates eversion of 8-oxoguanine by stabilizing several intermediate states, helping the rapidly sliding enzyme avoid full extrusion of every encountered base for interrogation Escherichia coli
physiological function Fpg selectively facilitates eversion of 8-oxoguanine by stabilizing several intermediate states, helping the rapidly sliding enzyme avoid full extrusion of every encountered base for interrogation Geobacillus stearothermophilus