Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000181 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | 15°C | Escherichia coli | |
0.0000222 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | 17.5°C | Escherichia coli | |
0.0000296 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | 20°C | Escherichia coli | |
0.0000369 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | 22.5°C | Escherichia coli | |
0.0000466 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
DNA containing ring-opened N7-methylguanine + H2O = deguanylated DNA + 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine | formation of the transition state complex from the reactants exhibits an overall free energy of 9.6 kcal/mol | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
13mer oligonucleotide duplex containing 8-oxoguanine + H2O | - |
Escherichia coli | 8-oxoguanine + oligonucleotide | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | 15°C | Escherichia coli | |
0.0106 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | 17.5°C | Escherichia coli | |
0.0148 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | 20°C | Escherichia coli | |
0.0202 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | 22.5°C | Escherichia coli | |
0.0273 | - |
13mer oligonucleotide duplex containing 8-oxoguanine | 25°C | Escherichia coli |