KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000013 | - |
DNA containing 8-hydroxyguanine residues | pH 7.5, 37°C | Escherichia coli | |
0.000038 | - |
DNA containing ring-opened N7-methylguanine residues | pH 7.5, 37°C | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains a single zinc finger motif near the C-terminus | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing 2,6-diamino-4-hydroxyformamidopyrimidine residues + H2O | Escherichia coli | repair of the major DNA lesions 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxyformamidopyrimidine formed by reactive oxidative species | 2,6-diamino-4-hydroxyformamidopyrimidine + DNA | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | Escherichia coli | repair of the major DNA lesions 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxyformamidopyrimidine formed by reactive oxidative species | 8-hydroxyguanine + DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
DNA containing ring-opened N7-methylguanine + H2O = deguanylated DNA + 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine | reaction mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA containing 2,6-diamino-4-hydroxyformamidopyrimidine residues + H2O | repair of the major DNA lesions 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxyformamidopyrimidine formed by reactive oxidative species | Escherichia coli | 2,6-diamino-4-hydroxyformamidopyrimidine + DNA | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | comparison of repair activities of human OGG1 and Escherichia coli Fpg, enzymes show distinct preferences for the base opposite 8-oxoguanine, mechanism via Schiff base intermediate | Escherichia coli | 8-hydroxyguanine + DNA | treatment of 7,8-dihydro-8-oxoguanine with Fpg results in delta-elimination products | ? | |
DNA containing 8-hydroxyguanine residues + H2O | readily incises dublexes with cytosine, thymine or guanine opposite, but much slower with adenine opposite 7,8-dihydro-8-oxoguanine, 2 activities: DNA-glycosylase and DNA nicking at abasic sites | Escherichia coli | 8-hydroxyguanine + DNA | treatment of 7,8-dihydro-8-oxoguanine with Fpg results in delta-elimination products | ? | |
DNA containing 8-hydroxyguanine residues + H2O | 7,8-dihydro-8-oxoguanine-DNA | Escherichia coli | 8-hydroxyguanine + DNA | treatment of 7,8-dihydro-8-oxoguanine with Fpg results in delta-elimination products | ? | |
DNA containing 8-hydroxyguanine residues + H2O | repair of the major DNA lesions 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxyformamidopyrimidine formed by reactive oxidative species | Escherichia coli | 8-hydroxyguanine + DNA | - |
? | |
DNA containing ring-opened N7-methylguanine residues + H2O | comparison of repair activities of human OGG1 and Escherichia coli Fpg, almost no paired base-dependent repair, effect of sequence context on repair efficiency, mechanism via Schiff base intermediate | Escherichia coli | 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA | treatment of me-Fapy with Fpg results in delta-elimination products | ? | |
additional information | human OGG1 and Escherichia coli Fpg are structurally unrelated enzymes with different catalytic residues, OGG1: Lys-249 and Asp-268 in the hairpin-helix-hairpin-GDP motif are involved in the glycosylase/AP lyase activity, Fpg: uses a proline residue in the N-terminal region for catalysis | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 30000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
DNA containing 8-hydroxyguanine residues | pH 7.5, 37°C | Escherichia coli | |
0.085 | - |
DNA containing ring-opened N7-methylguanine residues | pH 7.5, 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |