Protein Variants | Comment | Organism |
---|---|---|
K57G | study of the effect of the mutation on the structure dynamics, mutant with decreased 8-hydroxyguanine-DNA glycosylase activity | Escherichia coli |
P2E | study of the effect of the mutation on the structure dynamics, mutation causes complete loss of DNA glycosylase/beta-lyase activity and induces a conformational change leading to a more rigid globular structure than wild-type, K57G and P2G Fpg | Escherichia coli |
P2G | study of the effect of the mutation on the structure dynamics, mutant with complete loss of beta-lyase and partial loss of DNA glycosylase activity | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | possesses a zinc finger | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | Escherichia coli | involved in the repair of oxidized purines generated in the genome by endogenous or exogenous oxidative stress | ? | - |
? | |
DNA + H2O | Escherichia coli BH20 | involved in the repair of oxidized purines generated in the genome by endogenous or exogenous oxidative stress | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
containing pFPG220 | - |
Escherichia coli BH20 | - |
containing pFPG220 | - |
Purification (Comment) | Organism |
---|---|
wild-type and K57G, P2G, P2E mutant Fpg | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + H2O | removes oxidized purines from oxidatively damaged DNA | Escherichia coli | ? | - |
? | |
DNA + H2O | enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity | Escherichia coli | ? | - |
? | |
DNA + H2O | involved in the repair of oxidized purines generated in the genome by endogenous or exogenous oxidative stress | Escherichia coli | ? | - |
? | |
DNA + H2O | removes oxidized purines from oxidatively damaged DNA | Escherichia coli BH20 | ? | - |
? | |
DNA + H2O | enzyme has N-glycosylase and apurinic/apyrimidinic lyase activity | Escherichia coli BH20 | ? | - |
? | |
DNA + H2O | involved in the repair of oxidized purines generated in the genome by endogenous or exogenous oxidative stress | Escherichia coli BH20 | ? | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | - |
Escherichia coli | 8-hydroxyguanine + DNA | - |
? | |
DNA containing 8-hydroxyguanine residues + H2O | - |
Escherichia coli BH20 | 8-hydroxyguanine + DNA | - |
? | |
DNA containing ring-opened N7-methylguanine residues + H2O | - |
Escherichia coli | 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA | - |
? | |
DNA containing ring-opened N7-methylguanine residues + H2O | - |
Escherichia coli BH20 | 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine + DNA | - |
? |