Literature summary for 3.2.2.22 extracted from

Reyes, A.G.; Geukens, N.; Gutschoven, P.; De Graeve, S.; De Mot, R.; Mejia Alvarez, A.; Anne, J.
Streptomyces coelicolor genome encodes a type I ribosome-inactivating protein (2010), Microbiology, 156, 3021-3030.

Cloned(Commentary)

Cloned (Comment)	Organism
gene ripsc, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain TG1, functional expression of Hi6-tagged enzyme in Escherichia coli strain BL21(DE3) as recombinant protein of about 30 kDa in inclusion bodies. Expression in Streptomyces lividans and Escherichia coli, RIPsc overproduction results in dramatic decrease of the growth rate. In addition, intracellular production is deleterious for Saccharomyces cerevisiae. Detailed overview	Streptomyces coelicolor

Cloned (Comment)

Organism

gene ripsc, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain TG1, functional expression of Hi6-tagged enzyme in Escherichia coli strain BL21(DE3) as recombinant protein of about 30 kDa in inclusion bodies. Expression in Streptomyces lividans and Escherichia coli, RIPsc overproduction results in dramatic decrease of the growth rate. In addition, intracellular production is deleterious for Saccharomyces cerevisiae. Detailed overview

Streptomyces coelicolor

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Streptomyces coelicolor	the enzyme displays N-glycosidase activity causing specific rRNA depurination. It has antifungal and cytotoxic effects and inhibits cell growth, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces coelicolor	-	gene ripsc	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant functional His6-tagged enzyme, solubilized from Escherichia coli inclusion bodies, by nickel affinity chromatography and gel filtration	Streptomyces coelicolor

Renatured (Commentary)

Renatured (Comment)	Organism
recombinant functional His6-tagged enzyme from Escherichia coli inclusion bodies, method, overview	Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	the enzyme displays N-glycosidase activity causing specific rRNA depurination. It has antifungal and cytotoxic effects and inhibits cell growth, overview	Streptomyces coelicolor	?	-	?
additional information	the enzyme displays N-glycosidase activity causing specific rRNA depurination. Purified RIPsc protein displays strong protein translation inhibitory activity	Streptomyces coelicolor	?	-	?

Synonyms

Synonyms	Comment	Organism
RIP	-	Streptomyces coelicolor
SCO7092	-	Streptomyces coelicolor
type I ribosome-inactivating protein	-	Streptomyces coelicolor