Application | Comment | Organism |
---|---|---|
analysis | facile analysis of RIP catalytic activity will have applications in plant toxin detection, inhibitor screens, mechanistic analysis of depurinating agents on oligonucleotides and intact ribosomes, and in cancer immunochemotherapy | Ricinus communis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Ricinus communis | |
0.00006 | - |
yeast 60S rRNA | pH 4.0 | Ricinus communis | |
0.0001 | - |
rabbit reticulocyte 80S rRNA | pH 4.0 | Ricinus communis | |
0.0022 | - |
stem-loop substrate A-10 | pH 4.0 | Ricinus communis | |
0.0103 | - |
A-14 2-dA stem-loop RNA/DNA hybrid | pH 4.0 | Ricinus communis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ricinus communis | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Ricinus communis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
A-14 2-dA stem-loop RNA/DNA hybrid + H2O | - |
Ricinus communis | ? | - |
? | |
additional information | ricin toxin A-chain catalyzes the hydrolytic depurination of a single base from a GAGA tetraloop of eukaryotic ribosomal RNA to release a single adenine from the sarcin-ricin loop. It catalyzes depurination of 80S rabbit reticulocyte ribosomes and 60S yeast ribosomes by RTA, substrates are stem-loop substrate A-10 and A-14 2-dA stem-loop RNA/DNA hybrid, in general 6mer to 18mer stem-loop DNA and RNA 28S SRL mimic oligonucleotides. Development of a high-sensitive quantitative adenine detection method for enzyme activity measurement through coupling of RIP activity with adenine phosphoribosyl transferase, EC 2.4.2.7, and pyruvate orthophosphate dikinase, EC 2.7.9.1, in a colimetric assay, discontinous and continouos formats, optimization, overview. Deoxyadenosine at the depurination site, GdAGA, of RNA stem-loop oligonucleotides increases the catalytic efficiency by RTA a factor of about 4 | Ricinus communis | ? | - |
? | |
rabbit reticulocyte 80S rRNA + H2O | - |
Ricinus communis | ? | - |
? | |
stem-loop substrate A-10 + H2O | - |
Ricinus communis | ? | - |
? | |
yeast 60S rRNA + H2O | - |
Ricinus communis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribosome-inactivating proteins | - |
Ricinus communis |
ricin | - |
Ricinus communis |
ricin toxin A-chain | - |
Ricinus communis |
RIP | - |
Ricinus communis |
RTA | - |
Ricinus communis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.078 | - |
stem-loop substrate A-10 | pH 4.0 | Ricinus communis | |
18.5 | - |
A-14 2-dA stem-loop RNA/DNA hybrid | pH 4.0 | Ricinus communis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
the pH 4.0 catalytic optimum of RTA on 6mer to 18mer stem-loop DNA and RNA 28S SRL mimic oligonucleotides is known to result from the protonation of two ionizable residues on substrate and/or RTA | Ricinus communis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
RTA is inactive at pH 7.0 | Ricinus communis |
General Information | Comment | Organism |
---|---|---|
malfunction | the enzyme causes inhibition of protein synthesis by loss of elongation factor binding resulting in cell death | Ricinus communis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | catalysis of 80S ribosome by RTA approaches the diffusion rate limit for enzymatic reactions | Ricinus communis |