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Literature summary for 3.2.2.22 extracted from
- A functional quantitative polymerase chain reaction assay for ricin, Shiga toxin, and related ribosome-inactivating proteins (2010), Anal. Biochem., 396, 204-211.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | inhibits at 0.1-0.5% | Ricinus communis |  |
| Tween-80 | inhibits at 0.1-0.5% | Ricinus communis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Ricinus communis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| commercial preparation | - |
Escherichia coli | - |
| commercial preparation | - |
Ricinus communis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate is 28S rRNA from RAW264.7 cells. RIPs remove a specific adenine from rRNA. Development of an assay method involving reverse transcriptase, evaluation, detailed overview | Escherichia coli | ? | - |
? | |
| additional information | substrate is 28S rRNA from RAW264.7 cells. RIPs remove a specific adenine from rRNA. Development of an assay method involving reverse transcriptase,evaluation, detailed overview | Ricinus communis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ricin | - |
Ricinus communis |
| Ricinus communis agglutinin II | - |
Ricinus communis |
| Shiga toxin | - |
Escherichia coli |
| Shiga toxin-1 | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
assay at | Escherichia coli |
| 55 | - |
assay at | Ricinus communis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 80 | activity range, profile, overview | Ricinus communis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Escherichia coli |
| 5 | - |
assay at | Ricinus communis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | 5.3 | narrow activity range, profile, overview | Escherichia coli |
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Release 2023.1 (January 2023)
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