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Literature summary for 3.2.2.22 extracted from

  • Nagasawa, Y.; Fujii, K.; Yoshikawa, T.; Kobayashi, Y.; Kondo, T.
    Pokeweed antiviral protein region Gly209-Lys225 is critical for RNA N-glycosidase activity of the prokaryotic ribosome (2008), Phytochemistry, 69, 1653-1660.
    View publication on PubMed
Search for more literature for 3.2.2.22

Cloned(Commentary)

Cloned (Comment) Organism
plasmid pMAL-c2 to amplify pokeweed antiviral protein and karasurin-A by PCR, expression in Escherichia coli XL1-Blue cells with expression plasmid pGEX-6P-1 Phytolacca americana
plasmid pMAL-c2 to amplify pokeweed antiviral protein and karasurin-A by PCR, expression in Escherichia coli XL1-Blue cells with expression plasmid pGEX-6P-1 Trichosanthes kirilowii

Protein Variants

Protein Variants Comment Organism
additional information chimera proteins of 3 domains of pokeweed antiviral protein (PAP) and karasurin-A (N-terminal 1-74, central 75-177, C-terminal 178-262), C-terminal end determines determines specificity to eukaryotic or eukaryotic and prokaryotic ribosomes, PAP-region 209-225 is required for activity towards prokaryotic ribosomes Phytolacca americana
additional information chimera proteins of 3 domains of pokeweed antiviral protein and karasurin-A (N-terminal 1-74, central 75-177, C-terminal 178-262), C-terminal end determines determines specificity to eukaryotic or eukaryotic and prokaryotic ribosomes, PAP-region 209-225 is required for activity towards prokaryotic ribosomes Trichosanthes kirilowii

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Trichosanthes kirilowii karasurin-A shows RNA N-glycosidase activity only towards eukaryotic ribosomes while pokeweed antiviral protein is active towards eukaryotic and prokaryotic ribosomes, removal of specific adenine from alpha-sarcin/ricin loop of the large rRNA, substrate specificity (eukaryotic/prokaryotic) determined by C-terminus residues 209-225 ?
-
 ?
additional information Phytolacca americana PAP shows RNA N-glycosidase activity towards eukaryotic and prokaryotic ribosomes, karasurin-A only towards eukaryotic ribosomes, removal of specific adenine from alpha-sarcin/ricin loop of the large rRNA, substrate specificity (eukaryotic/prokaryotic) determined by C-terminus residues 209-225 ?
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 ?

Organism

Organism UniProt Comment Textmining
Phytolacca americana
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 pokeweed
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Trichosanthes kirilowii P24478 var. japonica
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Purification (Commentary)

Purification (Comment) Organism
cell centrifugation, washing, lysozyme application, sonication, centrifugation, supernatants applied to GSTrap FF column, elution with sonication buffer and 10 mM reduced glutathione, desalted, concentrated and glutathione S-transferase (GST) cleaved off with PreScission Protease Phytolacca americana
cell centrifugation, washing, lysozyme application, sonication, centrifugation, supernatants applied to GSTrap FF column, elution with sonication buffer and 10 mM reduced glutathione, desalted, concentrated and glutathione S-transferase (GST) cleaved off with PreScission Protease Trichosanthes kirilowii

Source Tissue

Source Tissue Comment Organism Textmining
leaf Phytolacca americana Phytolacca americana
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root
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 Trichosanthes kirilowii
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information karasurin-A shows RNA N-glycosidase activity only towards eukaryotic ribosomes while pokeweed antiviral protein is active towards eukaryotic and prokaryotic ribosomes, removal of specific adenine from alpha-sarcin/ricin loop of the large rRNA, substrate specificity (eukaryotic/prokaryotic) determined by C-terminus residues 209-225 Trichosanthes kirilowii ?
-
 ?
additional information PAP shows RNA N-glycosidase activity towards eukaryotic and prokaryotic ribosomes, karasurin-A only towards eukaryotic ribosomes, removal of specific adenine from alpha-sarcin/ricin loop of the large rRNA, substrate specificity (eukaryotic/prokaryotic) determined by C-terminus residues 209-225 Phytolacca americana ?
-
 ?
RNA + H2O ribosomes from rat liver (eukaryotic) and Escherichia coli JM109 (prokaryotic), 37°C, 25 mM Tris-HCl buffer, pH 7.6 with 50 mM KCl and 5 mM MgCl2 Phytolacca americana ?
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 ?
RNA + H2O ribosomes from rat liver (eukaryotic) and Escherichia coli JM109 (prokaryotic), 37°C, 25 mM Tris-HCl buffer, pH 7.6 with 50 mM KCl and 5 mM MgCl2 Trichosanthes kirilowii ?
-
 ?

Synonyms

Synonyms Comment Organism
karasurin-A KRN recombinantly combined with pokeweed antiviral protein (PAP) Trichosanthes kirilowii
PAP recombinantly combined with karasurin-A (KRN) of Trichosanthes kirilowii Phytolacca americana
pokeweed antiviral protein
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 Phytolacca americana