Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) pLysS | Trichosanthes kirilowii |
Crystallization (Comment) | Organism |
---|---|
in complex with 11-mer of P-protein C-terminal end (c11-P) | Trichosanthes kirilowii |
Protein Variants | Comment | Organism |
---|---|---|
K173A/R174A/K177A | no ribosome interaction, drastically reduced N-glycosidase acitivity | Trichosanthes kirilowii |
V232K/N236D | 17-fold reduced activity compared to wild-type, no ribosome interaction, V232K is at mouth of hydrophobic pocket, where the LF motif of c11-P binds, expected to block the access of the P-protein to the pocket, N236D substitution removes hydrogen bond between the amide of Asn236 and the backbone carbonyl of Leu9 of c11-P | Trichosanthes kirilowii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Trichosanthes kirilowii | depurination of specific adenine at sarcin-ricin loop of 28S rRNA, preventing the binding of elongation factors to the GTPase activation centre of the ribosome | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichosanthes kirilowii | P09989 | medicinal plant with anti-tumor and anti-HIV properties | - |
Purification (Comment) | Organism |
---|---|
Hi-Trap CM-Sepharose column followed by Hi-Trap SP-Sepharose column with 20 mM phosphate buffer, pH 6.7 | Trichosanthes kirilowii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | depurination of specific adenine at sarcin-ricin loop of 28S rRNA, preventing the binding of elongation factors to the GTPase activation centre of the ribosome | Trichosanthes kirilowii | ? | - |
? | |
rRNA + H2O | depurination of A-4324 of 28S rRNA, inhibition of protein synthesis in a nuclease-untreated rabbit reticulocyte lysate | Trichosanthes kirilowii | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
trichosanthin | TCS | Trichosanthes kirilowii |