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Literature summary for 3.2.2.22 extracted from

  • Sikriwal, D.; Ghosh, P.; Batra, J.K.
    Ribosome inactivating protein saporin induces apoptosis through mitochondrial cascade, independent of translation inhibition (2008), Int. J. Biochem. Cell Biol., 40, 2880-2888.
    View publication on PubMed
Search for more literature for 3.2.2.22

Cloned(Commentary)

Cloned (Comment) Organism
Escherichia coli BL21 and TKB1 Saponaria officinalis

Protein Variants

Protein Variants Comment Organism
R179A loss of RNA N-glycosidase and DNA fragmentation activities, no significant increase of caspase-3 and caspase-9 activity, 22% less cytotoxic than wild-type Saponaria officinalis
W208A RNA N-glycosidase activity retained, but total abolishment of DNA fragmentation activity, no significant increase of caspase-3 and caspase-9 activity, 5% less cytotoxic than wild-type Saponaria officinalis
Y16A almost complete loss of RNA N-glycosidase activity not affecting DNA laddering activity, about 12fold increase of caspase-3 activity and 6-fold increase of caspase-9 activity comparable to wild-type, 7% less cytotoxic than wild-type Saponaria officinalis

Organism

Organism UniProt Comment Textmining
Saponaria officinalis
-
 soapwort
-

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Saponaria officinalis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 no effect on protein synthesis below 4 microM saporin-6, 20% inhibition with 4 microM after 24 h, at 10 and 18 h ID50 greater than 8 microM, at 24 h 7 microM Saponaria officinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
rRNA + H2O removal of adenine residue from rRNA loop, caspase-dependent apoptosis induction in human histiocytic lymphoma U937 cells via mitochondiral pathway Saponaria officinalis ?
-
 ?

Synonyms

Synonyms Comment Organism
saporin-6 isoform with highest catalytic and cytotoxic activity Saponaria officinalis