Cloned (Comment) | Organism |
---|---|
Escherichia coli BL21 and TKB1 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
Y1477F | binding site for tyrosine kinase Syk at clathrin chain | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
transfected Ramos cell cultures | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | phosphorylation of clathrin heavy chain, mediated by tyrosine kinase Syk activity after binding to its cell receptor Gb3 | Escherichia coli |
Purification (Comment) | Organism |
---|---|
sonicated, cleared lysate of cell culture in 20 mM Tris buffer, pH 8.0 with 100 mM NaCl, 1 mM Na-vanadate and protease inhibitors (GST-buffer), incubated with glutathione-Sepharose beads, transferred to column, washed with 0.1 M Tris buffer, pH 7.8 with 0.5 M NaCl, with GST-buffer containing 0.35% Triton X-100, and GST-buffer alone | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
Shiga toxin | - |
Escherichia coli |
Stx | - |
Escherichia coli |