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Literature summary for 3.2.2.22 extracted from

  • Wong, J.H.; Wang, H.X.; Ng, T.B.
    Marmorin, a new ribosome inactivating protein with antiproliferative and HIV-1 reverse transcriptase inhibitory activities from the mushroom Hypsizigus marmoreus (2008), Appl. Microbiol. Biotechnol., 81, 669-674.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
9567
-
MALDI-TOF MS, SDS-PAGE Hypsizygus marmoreus
10000
-
1 * 10000, SDS-PAGE Hypsizygus marmoreus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
28S rRNA + H2O Hypsizygus marmoreus depurination at A4324, removal of adenine from rRNA, inactivating ribosomal function in translation 28S rRNA fragment + ?
-
?

Organism

Organism UniProt Comment Textmining
Hypsizygus marmoreus
-
mushroom, comparison with ribosome inhibitory proteins of bitter gourd, hairy gourd, ridge gourd, garden pea, Flammulina velutipes (mushroom with velutin)
-

Purification (Commentary)

Purification (Comment) Organism
centrifugation of homogenized fruiting bodies followed by ion exchange chromatography on a diethylaminoethyl-cellulose column in 10 mM Tris-HCl buffer, pH 7, Affi-gel blue gel column chromatography of unabsorbed protein fraction (with translation-inhibiting activity) in 10 mM Tris-HCl buffer, pH 7 yields bound marmorin Hypsizygus marmoreus

Source Tissue

Source Tissue Comment Organism Textmining
mushroom body fresh fruiting Hypsizygus marmoreus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
marmorin inhibits proliferation of hepatoma Hep G2 cells (IC50 = 0.00015 mM), breast cancer MCF-7 cells (IC50 = 0.005 mM), HIV-1 reverse transcriptase activity (IC50 = 0.03 mM), and translation in the rabbit reticulocyte lysate system (IC50 = 0.0000007 mM), no inhibitory action on embryonic liver WRL-68 cells, no antifungal, mitogenic/antimitogenic, protease, protease inhibitory, RNase, and nitric oxide-inducing activities Hypsizygus marmoreus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
28S rRNA + H2O depurination at A4324, removal of adenine from rRNA, inactivating ribosomal function in translation Hypsizygus marmoreus 28S rRNA fragment + ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 10000, SDS-PAGE Hypsizygus marmoreus

Synonyms

Synonyms Comment Organism
marmorin
-
Hypsizygus marmoreus
ribosome inactivating protein
-
Hypsizygus marmoreus