Application | Comment | Organism |
---|---|---|
diagnostics | identification of toxin with Vero-2dEGFP assay, threshold for toxin detection 10 picog/ml, and identification of toxin inhibitors, 10% glycerol enhances fluorescence signal | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
grape pomace extract | i.e. grape skin extract, dilutions of 0.5 mg/ml extract, 200fold greater EC50 (50% effective concentration) | Escherichia coli | |
grape seed extract | dilutions of 0.5 mg/ml extract, 6700fold greater EC50(50% effective concentration) | Escherichia coli | |
additional information | red wine concentrate (dilutions of 1 mg/ml) and caffeic acid (dilutions of 0.5 mg/ml) without inhibitory function in the assay | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
O157:H7 containing Shiga toxins type 1 and 2 (Stx1, Stx2), strains RM1697 (Stx1 + Stx2 producing O157 strain), RM4876 (non-Stx-producing o157 strain), RM5034 (non-pathogenic strain) | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
about 90% retained protein synthesis activity in assay cells and about 70% fluorescence by 0.001 ng/ml Stx2, about 50% activity and fluorescence by 0.1 ng/ml Stx2, about 30% activity and fluorescence by 100 ng/ml Stx2, Shiga toxin type 1 and 2 producing cell culture of strain RM1697 has similar dose-response curve as Stx2 | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
Shiga toxin | - |
Escherichia coli |
Shiga toxin type 1 | - |
Escherichia coli |
Shiga toxin type 2 | - |
Escherichia coli |
Stx1 | - |
Escherichia coli |
Stx2 | - |
Escherichia coli |