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Literature summary for 3.2.2.22 extracted from

  • Roday, S.; Saen-oon, S.; Schramm, V.L.
    Vinyldeoxyadenosine in a sarcin-ricin RNA loop and its binding to ricin toxin A-chain (2007), Biochemistry, 46, 6169-6182.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.2.22

Crystallization (Commentary)

Crystallization (Comment) Organism
dynamics calculations suggest that steric factors cause the nucleoside to bind in an orientation where the enzyme destabilizes the formation of the oxacarbeniumion and thus precludes catalysis Ricinus communis

Inhibitors

Inhibitors Comment Organism Structure
8-vinyl-2'-deoxyadenosine containing 10-mer stem-tetraloop RNA i.e. 8VdA-10, active site analogue, no substrate. Role of residue R180 in oxacarbenium ion destabilization, adenine is released from a second site within the molecule Ricinus communis

Organism

Organism UniProt Comment Textmining
Phytolacca americana
-
 isoform pokeweed antiviral protein, i.e. PAP
-
Ricinus communis P02879
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
8-vinyl-2'-deoxyadenosine containing 10-mer stem-tetraloop RNA + H2O
-
 Phytolacca americana 8-vinyladenine + ? slow ?
additional information enzyme catalyzes the release of two adenine residues Ricinus communis ?
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0024
-
 8-vinyl-2'-deoxyadenosine containing 10-mer stem-tetraloop RNA 37°C, pH 4.0 Ricinus communis