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Literature summary for 3.2.2.22 extracted from

  • Wang, L.C.; Kang, L.; Hu, T.M.; Wang, J.L.
    Abrin-a A chain expressed as soluble form in Escherichia coli from a PCR-synthesized gene is catalytically and functionally active (2004), Biochimie, 86, 327-333.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of His6-tagged optimized abricin A-chain in Escherichia coli strain BL21(DE3) Abrus precatorius

Protein Variants

Protein Variants Comment Organism
additional information optimization of the abricin A-chain by replacing rare codons with high-frequency ones, overview Abrus precatorius

Inhibitors

Inhibitors Comment Organism Structure
SDS inhibition at 0.5% Abrus precatorius

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme enters target cells via its cell surface receptor Abrus precatorius
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
2 * 30000, recombinant abricin A-chain, SDS-PAGE Abrus precatorius

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Abrus precatorius the cytotoxic enzyme irreversibly inactivates eukaryotic ribosomes in target cells, e.g. Sp2/0 cells and SMMC-7721 cells, by depurinating a key adenine residue from a highly conserved GAGA loop in 28S rRNA thereby inhibiting protein synthesis ?
-
?

Organism

Organism UniProt Comment Textmining
Abrus precatorius Q7DM12
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged optimized abricin A-chain from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Abrus precatorius

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Abrus precatorius
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
50% inhibition of L-leucine incorporation in a protein inhibition assay in a rabbit reticulocyte cell-free system Abrus precatorius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
28S RNA + H2O substrate from rat liver ribosomes, depurination of A4324, generation of a 420nt fragment, product determination using aniline coupling Abrus precatorius ?
-
?
additional information the cytotoxic enzyme irreversibly inactivates eukaryotic ribosomes in target cells, e.g. Sp2/0 cells and SMMC-7721 cells, by depurinating a key adenine residue from a highly conserved GAGA loop in 28S rRNA thereby inhibiting protein synthesis Abrus precatorius ?
-
?
additional information protein inhibition assay in a rabbit reticulocyte cell-free system with inhibition of L-leucine incorporation Abrus precatorius ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 30000, recombinant abricin A-chain, SDS-PAGE Abrus precatorius
More the A-chain exhibits the RNA N-glycosidase activity, while the B-chain mediates cell entry by binding to the target cell surface glycolipid receptor Abrus precatorius

Synonyms

Synonyms Comment Organism
ABRaA
-
Abrus precatorius
abrin
-
Abrus precatorius
abrin-a A chain
-
Abrus precatorius
More the enzyme belongs to the type-II-ribosome-inactivating protein family Abrus precatorius
RNA N-glycosidase
-
Abrus precatorius
type II ribosome-inactivating protein
-
Abrus precatorius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Abrus precatorius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Abrus precatorius