Cloned (Comment) | Organism |
---|---|
expression of His6-tagged optimized abricin A-chain in Escherichia coli strain BL21(DE3) | Abrus precatorius |
Protein Variants | Comment | Organism |
---|---|---|
additional information | optimization of the abricin A-chain by replacing rare codons with high-frequency ones, overview | Abrus precatorius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SDS | inhibition at 0.5% | Abrus precatorius |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme enters target cells via its cell surface receptor | Abrus precatorius | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
2 * 30000, recombinant abricin A-chain, SDS-PAGE | Abrus precatorius |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Abrus precatorius | the cytotoxic enzyme irreversibly inactivates eukaryotic ribosomes in target cells, e.g. Sp2/0 cells and SMMC-7721 cells, by depurinating a key adenine residue from a highly conserved GAGA loop in 28S rRNA thereby inhibiting protein synthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Abrus precatorius | Q7DM12 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged optimized abricin A-chain from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Abrus precatorius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | - |
Abrus precatorius | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
50% inhibition of L-leucine incorporation in a protein inhibition assay in a rabbit reticulocyte cell-free system | Abrus precatorius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
28S RNA + H2O | substrate from rat liver ribosomes, depurination of A4324, generation of a 420nt fragment, product determination using aniline coupling | Abrus precatorius | ? | - |
? | |
additional information | the cytotoxic enzyme irreversibly inactivates eukaryotic ribosomes in target cells, e.g. Sp2/0 cells and SMMC-7721 cells, by depurinating a key adenine residue from a highly conserved GAGA loop in 28S rRNA thereby inhibiting protein synthesis | Abrus precatorius | ? | - |
? | |
additional information | protein inhibition assay in a rabbit reticulocyte cell-free system with inhibition of L-leucine incorporation | Abrus precatorius | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 30000, recombinant abricin A-chain, SDS-PAGE | Abrus precatorius |
More | the A-chain exhibits the RNA N-glycosidase activity, while the B-chain mediates cell entry by binding to the target cell surface glycolipid receptor | Abrus precatorius |
Synonyms | Comment | Organism |
---|---|---|
ABRaA | - |
Abrus precatorius |
abrin | - |
Abrus precatorius |
abrin-a A chain | - |
Abrus precatorius |
More | the enzyme belongs to the type-II-ribosome-inactivating protein family | Abrus precatorius |
RNA N-glycosidase | - |
Abrus precatorius |
type II ribosome-inactivating protein | - |
Abrus precatorius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Abrus precatorius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Abrus precatorius |