Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant PAPs in Escherichia coli strain BL21(DE3) | Phytolacca americana |
Protein Variants | Comment | Organism |
---|---|---|
E176V | site-directed mutagenesis, inactive mutant showing no cytotoxicity | Phytolacca americana |
N70A | site-directed mutagenesis, the mutation delays the enzyme depurination activity on ribosomes and the onset of translation arrest, N70A PAP shows highly reduced cytotoxicity and destabilizes its own mRNA, but binds to cap and blocks cap-dependent translation | Phytolacca americana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the enzyme is synthesized as inactive precursor protein, accumulated in the endoplasmic reticulum associated to membranes, and needs to cleaved to the mature active form in the endoplasmic reticulum of the targeted cell, the mature form is retro-translocated from the ER to the cytosol where it is accumulated and escapes degradation, residues E176 and N70 are essential for catalytic activity | Phytolacca americana | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
rRNA + H2O | Phytolacca americana | PAP causes translation arrest and is cytotoxic in targeted cells | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phytolacca americana | P10297 | pokeweed | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the enzyme is synthesized as inactive precursor protein and needs to cleaved to the mature active form in the endoplasmic reticulum of the targeted cell, the mature form is retro-translocated from the ER to the cytosol where it escapes degradation | Phytolacca americana |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant PAPs from Escherichia coli strain BL21(DE3) by m7G affinity chromatography | Phytolacca americana |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
A4324 in 28S rRNA + H2O = deadenylated 28S rRNA + adenine | N70 of PAP is required for depurination catalytic activity, N70 facilitates depurination in trans | Phytolacca americana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Phytolacca americana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
rRNA + H2O | PAP causes translation arrest and is cytotoxic in targeted cells | Phytolacca americana | ? | - |
? | |
rRNA + H2O | pokeweed antiviral protein binds to ribosomes and depurinates a specific adenine in the highly conserved alpha-sarcin/ricin loop of the large subunit rRNA | Phytolacca americana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Phytolacca americana |
Synonyms | Comment | Organism |
---|---|---|
PAP | - |
Phytolacca americana |
pokeweed antiviral protein | - |
Phytolacca americana |
type I ribosome-inactivating protein | - |
Phytolacca americana |
type I RIP | - |
Phytolacca americana |