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Literature summary for 3.2.2.22 extracted from
- Purification, characterization and molecular cloning of trichoanguin, a novel type I ribosome-inactivating protein from the seeds of Trichosanthes anguina (1999), Biochem. J., 328, 211-219.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | trichoanguin examined for preparation of immunotoxins, with a great potential for application as an effective chemotherapeutic agent for the treatment of various cancers or AIDS | Trichosanthes anguina |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA cloned and transformed into Escherichia coli JM109 | Trichosanthes anguina |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
SDS-PAGE | Trichosanthes anguina |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| rRNA + H2O | Trichosanthes anguina | - |
? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trichosanthes anguina | - |
snake gourds | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Trichosanthes anguina |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Trichosanthes anguina |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Trichosanthes anguina | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activity 14800000 units, 1 unit the amount of enzyme necessary to inhibit protein synthesis by 50% in 1 ml rabbit reticulocyte lysate | Trichosanthes anguina |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| rRNA + H2O | - |
Trichosanthes anguina | ? | - |
? | |
| rRNA + H2O | cleaves the N-glycosidic bond and removes adenine at A-4324 in 28 S rRNA when intact rat ribosomes are the substrate, cleaves the same N-glycosidic bond in naked 28 S rRNA | Trichosanthes anguina | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| trichoanguin | - |
Trichosanthes anguina |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Trichosanthes anguina | - |
- |
9.1 |
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Release 2023.1 (January 2023)
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