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Literature summary for 3.2.2.22 extracted from

  • Barbieri, L.; Valbonesi, P.; Gorini, P.; Pession, A.; Stirpe, F.
    Polynucleotide: adenosine glycosidase activity of saporin-L1: effect on DNA, RNA and poly(A) (1996), Biochem. J., 319, 507-513.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Na+ reaction with poly(A) and hdpDNA only at low ionic strength, no activity at 0.45 M NaCl Saponaria officinalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.065
-
Poly(A) pH 6.0, 30°C Saponaria officinalis
0.639
-
Poly(A) pH 7.8, 25°C Saponaria officinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
rRNA + H2O Saponaria officinalis
-
?
-
?

Organism

Organism UniProt Comment Textmining
Saponaria officinalis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saponaria officinalis

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Saponaria officinalis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Saponaria officinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity towards various adenine-conating non-polynucleotide compounds like cytokinins, cofactors or nucleotides, releases adenine from hsDNA, hdpDNA, tRNA Phe-specific from Saccharomyces cervisiae, globin (alpha + beta) mRNA from rabbit reticulocyte, genomic RNA from bacteriophage MS 2, rRNA 16S and 23S from Escherichia coli, poly(A)- RNA from Bryonica dioica, genomic RNA from tobacco mosaic virus and artichoke mottled crinkle virus Saponaria officinalis ?
-
?
poly(A) + H2O
-
Saponaria officinalis adenine + ?
-
?
rRNA + H2O
-
Saponaria officinalis ?
-
?
rRNA + H2O cleaves the N-glycosidic bond and removes adenine at A-4324 in 28 S rRNA when intact rat ribosomes are the substrate, cleaves the same N-glycosidic bond in naked 28 S rRNA Saponaria officinalis ?
-
?
supercoiled double-stranded DNA + H2O saporin-L1 depurinates DNA extensively and releases adenine from all adenine-containing polynucleotides Saponaria officinalis nicked and linear forms of DNA
-
?

Synonyms

Synonyms Comment Organism
saporin-L1
-
Saponaria officinalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
optimum temperature for reaction with poly(A) Saponaria officinalis
50
-
optimum temperature for reaction with MS 2 RNA and hdpDNA Saponaria officinalis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 50
-
Saponaria officinalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
highest temperature compatible with enzyme activity Saponaria officinalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.02
-
Poly(A) pH 7.8, 25°C Saponaria officinalis
3.05
-
Poly(A) pH6.0, 30°C Saponaria officinalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
reaction rate with MS 2 RNA and hdpDNA decreases progressively at values above Saponaria officinalis
6
-
sharp increase of the reaction rate with poly(A) between pH 5.5 and pH 7.5 Saponaria officinalis
6.5
-
optimum for reaction with hdpDNA Saponaria officinalis

pH Range

pH Minimum pH Maximum Comment Organism
3.5 7.5 adenine realease from poly(A) and MS 2 RNA Saponaria officinalis

Cofactor

Cofactor Comment Organism Structure
additional information reaction proceeds without cofactor Saponaria officinalis