Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Na+ | reaction with poly(A) and hdpDNA only at low ionic strength, no activity at 0.45 M NaCl | Saponaria officinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
Poly(A) | pH 6.0, 30°C | Saponaria officinalis | |
0.639 | - |
Poly(A) | pH 7.8, 25°C | Saponaria officinalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
rRNA + H2O | Saponaria officinalis | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saponaria officinalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saponaria officinalis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Saponaria officinalis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saponaria officinalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity towards various adenine-conating non-polynucleotide compounds like cytokinins, cofactors or nucleotides, releases adenine from hsDNA, hdpDNA, tRNA Phe-specific from Saccharomyces cervisiae, globin (alpha + beta) mRNA from rabbit reticulocyte, genomic RNA from bacteriophage MS 2, rRNA 16S and 23S from Escherichia coli, poly(A)- RNA from Bryonica dioica, genomic RNA from tobacco mosaic virus and artichoke mottled crinkle virus | Saponaria officinalis | ? | - |
? | |
poly(A) + H2O | - |
Saponaria officinalis | adenine + ? | - |
? | |
rRNA + H2O | - |
Saponaria officinalis | ? | - |
? | |
rRNA + H2O | cleaves the N-glycosidic bond and removes adenine at A-4324 in 28 S rRNA when intact rat ribosomes are the substrate, cleaves the same N-glycosidic bond in naked 28 S rRNA | Saponaria officinalis | ? | - |
? | |
supercoiled double-stranded DNA + H2O | saporin-L1 depurinates DNA extensively and releases adenine from all adenine-containing polynucleotides | Saponaria officinalis | nicked and linear forms of DNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
saporin-L1 | - |
Saponaria officinalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
optimum temperature for reaction with poly(A) | Saponaria officinalis |
50 | - |
optimum temperature for reaction with MS 2 RNA and hdpDNA | Saponaria officinalis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | - |
Saponaria officinalis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
highest temperature compatible with enzyme activity | Saponaria officinalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.02 | - |
Poly(A) | pH 7.8, 25°C | Saponaria officinalis | |
3.05 | - |
Poly(A) | pH6.0, 30°C | Saponaria officinalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
reaction rate with MS 2 RNA and hdpDNA decreases progressively at values above | Saponaria officinalis |
6 | - |
sharp increase of the reaction rate with poly(A) between pH 5.5 and pH 7.5 | Saponaria officinalis |
6.5 | - |
optimum for reaction with hdpDNA | Saponaria officinalis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.5 | 7.5 | adenine realease from poly(A) and MS 2 RNA | Saponaria officinalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | reaction proceeds without cofactor | Saponaria officinalis |