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Literature summary for 3.2.2.22 extracted from

  • Ferreras, J.M.; Barbieri, L.; Girbes, T.; Battelli, M.G.; Rojo, M.A.; Arias, F.J.; Rocher, M.A.; Soriano, F.; Mendez, E.; Stirpe, F.
    Distribution and properties of major ribosome-inactivating proteins (28 S rRNA N-glycosidases) of the plant Saponaria officinalis L. (Caryophyllaceae) (1993), Biochim. Biophys. Acta, 1216, 31-42.
    View publication on PubMed
Search for more literature for 3.2.2.22

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ activity of 1 of 7 isoforms of saporin is dependent on magnesium concentration Saponaria officinalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27500 30100 7 major saporins, gel filtration Saponaria officinalis
30200
-
 1 * 30200, SO-R1, SDS-PAGE Saponaria officinalis
30900
-
 1 * 30900, SO-R2, SO-R3, and SO-S%, SDS-PAGE Saponaria officinalis
31600
-
 1 * 31600, SO-L1, SO-L2, and SO-S6, SDS-PAGE Saponaria officinalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
rRNA + H2O Saponaria officinalis
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Saponaria officinalis
-
 saporins
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 2 saporins present in roots, SO-R1 and SO-R3 are glycosylated Saponaria officinalis

Purification (Commentary)

Purification (Comment) Organism
7 major saporins Saponaria officinalis

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
 Saponaria officinalis
-
fruit
-
 Saponaria officinalis
-
leaf
-
 Saponaria officinalis
-
root
-
 Saponaria officinalis
-
seed
-
 Saponaria officinalis
-
stem
-
 Saponaria officinalis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activity 5000 units/mg of protein in old leaves, 6920 in young leaves, 6900 in stems, 37000 in roots, 640 in flower petals, 5400 in flower sepals, 2700 in flower ovaries, 1100 in flower pistils, 7000 in fruits skins, 365 in fruits cores, 17 in seeds at stage I, 135000 in seeds at stage II and III, and 120000 at stage IV, one unit is defined as the amount of protein necessary to inhibit protein synthesis by 50% in 1 ml of rabbit-reticulocyte lysate, specific activity of purified protein from seeds 633000, from roots 69200 Saponaria officinalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information all saporins are very active on cell-free translation systems derived from rabbit reticulocyte lysates, rat liver, Triticum aestivum, Cucumis sativus and Vicia sativa, but poor inhibitors of Escherichia coli translations systems , they inhibit protein synthesis in HeLa, BeWo and NB100 cells Saponaria officinalis ?
-
 ?
rRNA + H2O
-
 Saponaria officinalis ?
-
 ?
rRNA + H2O rat liver 28 S rRNA Saponaria officinalis ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 30200, SO-R1, SDS-PAGE Saponaria officinalis
monomer 1 * 31600, SO-L1, SO-L2, and SO-S6, SDS-PAGE Saponaria officinalis
monomer 1 * 30200-31600, 7 major saporins, SDS-PAGE Saponaria officinalis
monomer 1 * 30900, SO-R2, SO-R3, and SO-S%, SDS-PAGE Saponaria officinalis