Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
abasic-containing DNA | potent inhibitor | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
duplex oligonucleotide substrate containing ethenoadenine and hypoxanthine | at 37°C, in 50 mM Na-MES buffer (pH 6.1) | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
duplex oligonucleotide substrate containing ethenoadenine and hypoxanthine + H2O | - |
Escherichia coli | ethenoadenine + hypoxanthine + oligonucleotide | - |
? | |
additional information | the enzyme binds preferentially DNA ends, more tightly than hypoxanthine lesions, exhibits significant product inhibition under multiple-turnover conditions, and binds approximately 10fold more tightly to an abasic site than to a hypoxanthine lesion site | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-methyladenine DNA glycosylase II | - |
Escherichia coli |
AlkA | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00125 | - |
duplex oligonucleotide substrate containing ethenoadenine and hypoxanthine | at 37°C, in 50 mM Na-MES buffer (pH 6.1) | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.1 | - |
- |
Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
30 | - |
duplex oligonucleotide substrate containing ethenoadenine and hypoxanthine | at 37°C, in 50 mM Na-MES buffer (pH 6.1) | Escherichia coli |