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Literature summary for 3.2.2.21 extracted from

  • Zhao, B.; OBrien, P.J.
    Kinetic mechanism for the excision of hypoxanthine by Escherichia coli AlkA and evidence for binding to DNA ends (2011), Biochemistry, 50, 4350-4359.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
abasic-containing DNA potent inhibitor Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
duplex oligonucleotide substrate containing ethenoadenine and hypoxanthine at 37°C, in 50 mM Na-MES buffer (pH 6.1) Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
duplex oligonucleotide substrate containing ethenoadenine and hypoxanthine + H2O
-
Escherichia coli ethenoadenine + hypoxanthine + oligonucleotide
-
?
additional information the enzyme binds preferentially DNA ends, more tightly than hypoxanthine lesions, exhibits significant product inhibition under multiple-turnover conditions, and binds approximately 10fold more tightly to an abasic site than to a hypoxanthine lesion site Escherichia coli ?
-
?

Synonyms

Synonyms Comment Organism
3-methyladenine DNA glycosylase II
-
Escherichia coli
AlkA
-
Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00125
-
duplex oligonucleotide substrate containing ethenoadenine and hypoxanthine at 37°C, in 50 mM Na-MES buffer (pH 6.1) Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1
-
-
Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
30
-
duplex oligonucleotide substrate containing ethenoadenine and hypoxanthine at 37°C, in 50 mM Na-MES buffer (pH 6.1) Escherichia coli